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EMAGE:9123

Plcb4 phospholipase C, beta 4 ( MGI:107464)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9123 EMAGE:9123 EMAGE:9123 EMAGE:9123 EMAGE:9123
"Pseudo-wholemount" of euxassay_018633. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018633_01 euxassay_018633_02 euxassay_018633_03 euxassay_018633_04
EMAGE:9123 EMAGE:9123 EMAGE:9123 EMAGE:9123 EMAGE:9123
euxassay_018633_05 euxassay_018633_06 euxassay_018633_07 euxassay_018633_08 euxassay_018633_09
EMAGE:9123 EMAGE:9123 EMAGE:9123 EMAGE:9123 EMAGE:9123
euxassay_018633_10 euxassay_018633_11 euxassay_018633_12 euxassay_018633_13 euxassay_018633_14
EMAGE:9123 EMAGE:9123 EMAGE:9123 EMAGE:9123 EMAGE:9123
euxassay_018633_15 euxassay_018633_16 euxassay_018633_17 euxassay_018633_18 euxassay_018633_19
EMAGE:9123 EMAGE:9123 EMAGE:9123 EMAGE:9123 EMAGE:9123
euxassay_018633_20 euxassay_018633_21 euxassay_018633_22 euxassay_018633_23 euxassay_018633_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9123Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9123_wholemount_strong.wlz
9123_wholemount_moderate.wlz
9123_wholemount_weak.wlz
9123_wholemount_possible.wlz
9123_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9123_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50836
Entity Detected:Plcb4, phospholipase C, beta 4 ( MGI:107464)
Sequence:sense strand is shown

>T50836
AATACCATCATGGCCAAACCTTACGAATTTAACTGGCAGAAGGAAGTGCCCTCTTTCTTGCAAGAAGGAG
CAGTTTTTGACAGATACGAAGAGGAATCTTTTGTGTTTGAGCCCAACTGCCTCTTCAAAGTAGATGAATT
CGGCTTCTTCCTGACGTGGAAGAGTGAAGGCAAGGAAGGACAAGTGCTAGAATGTTCCCTCATCAACAGT
ATTCGCCAAGCAGCCATACCAAAGGATCCCAAAATCCTGGCTGCTCTCGAAGCTGTTGGAAAATCTGAAA
ATGATCTGGAAGGGCGGATATTGTGTGTCTGCAGCGGCACGGATCTGGTGAATATCGGCTTCACTTACAT
GGTGGCTGAAAATCCAGAAGTAACTAAGCAATGGGTAGAAGGCCTGAGATCGATCATTCACAACTTCAGG
GCAAACAACGTCAGTCCGATGACATGCCTCAAGAAACACTGGATGAAACTGGCCTTTCTGACCAACACAA
CTGGTAAAATCCCAGTGAGGAGTATCACTAGAACCTTCGCATCAGGGAAAACAGAAAAGGTGATCTTTCA
AGCCCTCAAGGAACTAGGTCTTCCCAGTGGAAAGAATGATGAAATTGAACCTGCTGCATTTACTTATGAA
AAGTTCTATGAACTGACACAAAAGATTTGTCCTCGGACAGATATAGAAGATCTTTTTAAAAAAATCAATG
GAGACAAAACTGATTATTTAACGGTAGACCAATTAGTGAGCTTTCTAAACGAACATCAGCGAGATCCTCG
GCTGAATGAAATTTTATTCCCATTTTATGATGCTAAAAGAGCAATGCAGATCATTGAAATGTATGAGCCT
GATGAAGAGCTGAA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AATACCATCATGGCCATGGCCAAACC; Reverse Primer - name:unspecified, sequence:TTCAGCTCTTCATCAGGCTC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9124 same embryo
 EMAGE:9127 same embryo
 EMAGE:9125 same embryo
 EMAGE:9126 same embryo
 EurExpress:euxassay_018633 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS