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EMAGE:914

Lmx1b LIM homeobox transcription factor 1 beta ( MGI:1100513)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:914
Fig 5L Cygan et al, 1997 [PMID:9362463] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
This is a vibratome section of a wildtype forelimb at 11.5dpc. This sample is double stained for Lmx1b (purple) and Fgf8 (orange). Only the Lmx1b data has been spatially mapped in this EMAGE entry.
Expression Pattern Description
Spatial Annotation:
EMAGE:914EMAGE:914Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
914_voxel_strong_3D_1.wlz
914_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:914_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future arm mesenchyme
detected detected
regionalExpression in the dorsal mesenchyme terminates at the level of Fgf8 expression in the ectoderm
handplate mesenchyme
detected detected
regionalExpression in the dorsal mesenchyme terminates at the level of Fgf8 expression in the ectoderm
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1196543
Entity Detected:Lmx1b, LIM homeobox transcription factor 1 beta ( MGI:1100513)
Notes:The probe used in this study by Cygan et al, 1997 [PMID:9362463] is described as "A plasmid containing the entire coding region of Lmx-1b was linearized with HindIII and transcribed with T7 polymerase to generate a 1.1 kb antisense probe". Exact probe end points are not defined.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount plus section of wholemount
Procedures
Fixation:4% paraformaldehyde
Embedding:2% agarose
General Information
Authors:Cygan et al, 1997 [PMID:9362463] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9362463] Cygan JA, Johnson RL, McMahon AP 1997 Novel regulatory interactions revealed by studies of murine limb pattern in Wnt-7a and En-1 mutants. Development (124):5021-32
Links:MGI:1933251 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI