EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:9179

Gad2 glutamic acid decarboxylase 2 ( MGI:95634)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017937. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017937_01	euxassay_017937_02	euxassay_017937_03	euxassay_017937_04

euxassay_017937_05	euxassay_017937_06	euxassay_017937_07	euxassay_017937_08	euxassay_017937_09

euxassay_017937_10	euxassay_017937_11	euxassay_017937_12	euxassay_017937_13	euxassay_017937_14

euxassay_017937_15	euxassay_017937_16	euxassay_017937_17	euxassay_017937_18	euxassay_017937_19

euxassay_017937_20	euxassay_017937_21	euxassay_017937_22	euxassay_017937_23	euxassay_017937_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9179_wholemount_strong.wlz

9179_wholemount_moderate.wlz

9179_wholemount_weak.wlz

9179_wholemount_possible.wlz

9179_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9179_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50430
Entity Detected:	Gad2, glutamic acid decarboxylase 2 ( MGI:95634)
Sequence:	sense strand is shown >T50430 CCTGACTGGAACATACAAACACGCACGAGCACTGGCATACGCAGACAGCACGTTTCCTGTCCCTGTGTGA CAACCACCCTCGTCGCGCTGCGCTCCAGGCCTCGCGCGGGTGGCCTTCTCCCGGCACACGTACTCGCACA CGCACGCACTCTCGTGCAGGGTCGAGCCAAAGCAGCTTACCACATCCACTTGGAGGCGACCAGCGCCAGT CTAGCAGAACCCATGGCGTCTCCTGGCTCCGGCTTTTGGTCCTTCGGATCTGAAGATGGCTCTGCGGATC CTGAGAACCCGGGCACAGCGAGAGCGTGGTGCCAGGTGGCCCAAAAGTTCACGGGCGGCATCGGAAACAA GCTGTGCGCTCTGCTCTATGGAGACTCCGGGAAGCCAGCGGAGGGCGGCGGGAGCGTGACCTCGCGGGCC GCCACCGGGAAGGTCGCCTGCACCTGCGACCAAAAACCCTGCAACTGCCCCAAAGGGGATGTCAACTACG CGTTTCTGCACGCAACAGACCTGCTGCCAGCGTGTGATGGAGAAAGGCCCACTCTGGCATTTCTACAAGA TGTTATGAACATTTTGCTTCAGTACGTGGTGAAAAGTTTCGATAGATCAACTAAAGTGATCGATTTCCAT TACCCCAATGAGCTTCTTCAAGAGTATAATTGGGAATTGGCAGACCAACCGCAAAATCTGGAGGAAATTT TGACGCATTGCCAAACAACTCTAAAATATGCAATTAAAACAGGGCATCCCCGATATTTTAATCAGCTGTC TACTGGATTGGATATGGTTGGATTAGCAGCAGACTGGTTGACATCAACAGCAAACACTAACATGTTCACC TATGAGATCGCCCCTGTATTTGTGCTGCTAGAATATGTTACACTAAAGAAAATGAGAGAAATCATTGGCT GGCCCGGGGGCTCTGGCGATGGAATCTTTTCTCCTGGTGGCGCCATCTCCAACATGTATGCCATGCTCAT TGCCCGCTATAAGATGTCTCCAGAAGTCAAGGAGAAGGGGATGGCAGCAGTGCCCAGGCTCATCGCGTTC ACATCAGAGCATAGTCACTTTTCTCTCAAGAAGGGAGCTGCAGCCTTAGGGATTGGAACAGACAGTGTGA TTCTGATTAAATGTGACGAGAGAGGGAAAATGATTCCATCTGATCTTGAAAGAAGAATCCTTGAAGTCAA ACAGGAAGGATTTGTTCCTTTCCTGGTGAGTGCCACAGCTGGAACCACCGTGTATGGGGCTTTTGATCCT CTCTTGGCTGTAGCTGACATCTGCAAAAAGTATAAGATCTGGATGCACGTGGATGCTGCTTGGGGTGGAG GGTTACTGATGTCCCGGAAACACAAGTGGAAGCTGAGTGGAGTAGAGAGGGCCAACTCTGTGACATGGAA TCCTCACAAGATGATGGGTGTCCCCTTGCAGTGTTCAGCTCTCCTGGTTAGAGAGGAGGGACTGATGCAG AGCTGCAACCAGATGCATGCTTCCTACCTCTTTCAGCAAGATAAGCACTATGACCTGTCCTATGACACAG GAGACAAGGCCTTGCAGTGTGGACGCCATGTGGATGTCTTTAAATTATGGCTCATGTGGAGAGCAAAGGG GACTACTGGGTTTGAGGCACACATTGATAAGTGTTTGGAGCTAGCAGAGTATTTATACACTATCATTAAA AACCGAGAAGGCTATGAAATGGTGTTTGATGGGAAGTCTCAACACACAAATGTCTGCTTCTGGTTTGTAC CTCCTAGTTTGCGCACTCTGGAAGACAATGAAGAGAGAATGAGCCGCCTCTCAAAGGTGGCGCCAGTGAT TAAAGCCAGAATGATGGAGTATGGGACCACAATGGTCAGCTACCAACCCTTAGGGGACAAGGTCAACTTC TTCCGCATGGTCATCTCAAACCCTGCAGCAACTCACCAAGACATTGACTTCCTTATTGAAGAAATCGAAC GCCTCGGACAAGATTTGTAATCACCTCACTCATCAAAACT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CCTGACTGGAACATACAAACACGC; Reverse Primer - name:unspecified, sequence:CCTTTTCACTGTACCCCAGAGAACC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9177 same embryo
	EMAGE:9180 same embryo
	EMAGE:9176 same embryo
	EMAGE:9178 same embryo
	EurExpress:euxassay_017937 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source