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EMAGE:9194

Ybx1 Y box protein 1 ( MGI:99146)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9194 EMAGE:9194 EMAGE:9194 EMAGE:9194 EMAGE:9194
euxassay_017966_01 euxassay_017966_02 euxassay_017966_03 euxassay_017966_04 euxassay_017966_05
EMAGE:9194 EMAGE:9194 EMAGE:9194 EMAGE:9194 EMAGE:9194
euxassay_017966_06 euxassay_017966_07 euxassay_017966_08 euxassay_017966_09 euxassay_017966_10
EMAGE:9194 EMAGE:9194 EMAGE:9194 EMAGE:9194 EMAGE:9194
euxassay_017966_11 euxassay_017966_12 euxassay_017966_13 euxassay_017966_14 euxassay_017966_15
EMAGE:9194 EMAGE:9194 EMAGE:9194 EMAGE:9194 EMAGE:9194
euxassay_017966_16 euxassay_017966_17 euxassay_017966_18 euxassay_017966_19 euxassay_017966_20
EMAGE:9194 EMAGE:9194 EMAGE:9194 EMAGE:9194
euxassay_017966_21 euxassay_017966_22 euxassay_017966_23 euxassay_017966_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9194Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9194_wholemount_strong.wlz
9194_wholemount_moderate.wlz
9194_wholemount_weak.wlz
9194_wholemount_possible.wlz
9194_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9194_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50766
Entity Detected:Ybx1, Y box protein 1 ( MGI:99146)
Sequence:sense strand is shown

>T50766
TCATCGCAACGAAGGTTTTGGGAACAGTCAAATGGTTCAATGTAAGGAACGGATACGGTTTCATCAACAG
GAATGACACCAAGGAAGACGTATTTGTACACCAGACTGCCATAAAGAAGAATAACCCCAGGAAGTACCTT
CGCAGTGTAGGCGATGGAGAGACTGTGGAGTTTGATGTTGTTGAAGGAGAAAAGGGTGCGGAGGCAGCAA
ATGTTACAGGCCCTGGTGGAGTTCCAGTTCAAGGCAGTAAATACGCAGCAGACCGTAACCATTATAGACG
CTATCCACGTCGTAGGGGTCCTCCACGCAATTACCAGCAAAATTACCAGAATAGTGAGAGTGGGGAAAAG
AACGAGGGATCGGAAAGCGCTCCTGAAGGCCAGGCCCAACAACGCCGGCCCTATCGCAGGCGAAGGTTCC
CACCTTACTACATGCGGAGACCCTATGCGCGTCGACCACAGTATTCCAACCCCCCTGTGCAAGGAGAAGT
GATGGAGGGTGCTGACAACCAGGGTGCAGGAGAGCAAGGTAGACCAGTGAGACAGAATATGTATCGCCCT
TACAGACCACGATTCCGAAGGGGCCCTCCTCGCCAAAGACAGCCTAGAGAGGATGGCAATGAAGAGGACA
AAGAAAATCAAGGAGATGAGACCCAAGGTCAGCAGCCACCTCAACGTCGGTATCGCCGAAACTTCAATTA
CCGACGCAGACGCCCAGAGAACCCTAAACCACAAGATGGCAAAGAGACAAAAGCAGCCGATCCACCAGCT
GAGAATTCGTCCGCTCCCGAGGCTGAGCAGGGCGGGGCTGAGTAAACGCCGGCTTA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TCACGCAACGAAGGTTTT; Reverse Primer - name:unspecified, sequence:TAAGCCGGCGTTTACTCAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9192 same embryo
 EMAGE:9196 same embryo
 EMAGE:9197 same embryo
 EMAGE:9193 same embryo
 EMAGE:9195 same embryo
 EurExpress:euxassay_017966 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS