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EMAGE:9205

Lef1 lymphoid enhancer binding factor 1 ( MGI:96770)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9205 EMAGE:9205 EMAGE:9205 EMAGE:9205 EMAGE:9205
"Pseudo-wholemount" of euxassay_018171. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018171_01 euxassay_018171_02 euxassay_018171_03 euxassay_018171_04
EMAGE:9205 EMAGE:9205 EMAGE:9205 EMAGE:9205 EMAGE:9205
euxassay_018171_05 euxassay_018171_06 euxassay_018171_07 euxassay_018171_08 euxassay_018171_09
EMAGE:9205 EMAGE:9205 EMAGE:9205 EMAGE:9205 EMAGE:9205
euxassay_018171_10 euxassay_018171_11 euxassay_018171_12 euxassay_018171_13 euxassay_018171_14
EMAGE:9205 EMAGE:9205 EMAGE:9205 EMAGE:9205 EMAGE:9205
euxassay_018171_15 euxassay_018171_16 euxassay_018171_17 euxassay_018171_18 euxassay_018171_19
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euxassay_018171_20 euxassay_018171_21 euxassay_018171_22 euxassay_018171_23 euxassay_018171_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9205Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9205_wholemount_strong.wlz
9205_wholemount_moderate.wlz
9205_wholemount_weak.wlz
9205_wholemount_possible.wlz
9205_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9205_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50619
Entity Detected:Lef1, lymphoid enhancer binding factor 1 ( MGI:96770)
Sequence:sense strand is shown

>T50619
TGAAGCCTCAACACGAACAGAGAAAGGAGCAGGAGCCCAAAAGACCTCATATTAAGAAGCCTCTGAATGC
TTTCATGTTATATATGAAAGAAATGAGAGCGAATGTCGTAGCTGAGTGCACGCTAAAGGAGAGTGCAGCT
ATCAACCAGATCCTGGGCAGAAGATGGCACGCCCTCTCCCGGGAAGAGCAGGCCAAATACTATGAACTAG
CACGGAAAGAGAGACAGCTACACATGCAGCTTTATCCAGGCTGGTCAGCGCGAGACAATTATGGCAAGAA
GAAGAAGAGGAAGAGAGAGAAGCTACAGGAGTCGACTTCAGGTACAGGTCCCAGAATGACAGCTGCCTAC
ATCTGAAACATGGTGGTAAGAGAAGCTCCTTCCCAACGTGCAAAGCCAAGGCAGCGACCCCAGGCCCTCT
TCTGGAGATGGAAGCTTGTTGAAACCCCAGACTGTCTCCACAGCTTGCCCGGCTGACCCCAAGGAACACT
GACAGCAACCTTACCCTGAGGTCACTGCTAGCGCTGACCCGAAGACACAGTCACTGCCACCTCTTCCTTC
TGTCGTCTACTGCAAGCGCCGACTTCCAAAAAGAAAGCCGAAAACGGTTGTTTCGGAAA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TGAAGCCTCAACACGAACAG; Reverse Primer - name:unspecified, sequence:TTTCCGAAACAACCGTTTTC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9206 same embryo
 EMAGE:9207 same embryo
 EMAGE:9203 same embryo
 EMAGE:9204 same embryo
 EurExpress:euxassay_018171 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS