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EMAGE:9215

Pvalb parvalbumin ( MGI:97821)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9215 EMAGE:9215 EMAGE:9215 EMAGE:9215 EMAGE:9215
"Pseudo-wholemount" of euxassay_018112. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018112_01 euxassay_018112_02 euxassay_018112_03 euxassay_018112_04
EMAGE:9215 EMAGE:9215 EMAGE:9215 EMAGE:9215 EMAGE:9215
euxassay_018112_05 euxassay_018112_06 euxassay_018112_07 euxassay_018112_08 euxassay_018112_09
EMAGE:9215 EMAGE:9215 EMAGE:9215 EMAGE:9215 EMAGE:9215
euxassay_018112_10 euxassay_018112_11 euxassay_018112_12 euxassay_018112_13 euxassay_018112_14
EMAGE:9215 EMAGE:9215 EMAGE:9215 EMAGE:9215 EMAGE:9215
euxassay_018112_15 euxassay_018112_16 euxassay_018112_17 euxassay_018112_18 euxassay_018112_19
EMAGE:9215 EMAGE:9215 EMAGE:9215 EMAGE:9215 EMAGE:9215
euxassay_018112_20 euxassay_018112_21 euxassay_018112_22 euxassay_018112_23 euxassay_018112_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9215Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9215_wholemount_strong.wlz
9215_wholemount_moderate.wlz
9215_wholemount_weak.wlz
9215_wholemount_possible.wlz
9215_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9215_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50788
Entity Detected:Pvalb, parvalbumin ( MGI:97821)
Sequence:sense strand is shown

>T50788
TCTGCTCATCCAAGTTGCAGGATGTCGATGACAGACGTGCTCAGCGCTGAGGACATCAAGAAGGCGATAG
GAGCCTTTGCTGCTGCAGACTCCTTCGACCACAAAAAGTTCTTCCAGATGGTGGGCCTGAAGAAAAAGAA
CCCGGATGAGGTGAAGAAGGTGTTCCATATTCTGGACAAAGACAAAAGTGGCTTCATTGAGGAGGATGAG
CTGGGGTCCATTCTGAAGGGCTTCTCCTCAGATGCCAGAGACTTGTCTGCTAAAGAAACAAAGACGCTTC
TGGCCGCTGGAGACAAGGATGGGGACGGCAAGATTGGGGTTGAAGAATTCTCCACTCTGGTGGCTGAAAG
CTAAGTGGCGCTGACTGCTTGGGTCCCCCACCTCTCCATCCCCAACGCCCCATCTCAGCCCTTCTCGCGG
CCCTCCTGAGTTTCTGTTCAGTTTGTTTGTGTTATTTTTTACTCCCCCATCCTCTATGGCCCTCGGATGA
CGCCATTCTTCTGGAAATGCTGGAGAAACAATAAAGGCTGTACCAATCTGACACCACCTGTAGGGAGGAC
CCAGGCCTGGCAGGGTGTTGGTTTGGCAAGTTTTTTTTCTTTCTTTTTAGGGCAGTGGGGGTATAGTAGA
AAAAGTGAGATAAGTCAAAGGACAACGCCCCGATATCTCCTGCCTGCTTGGTACTGAGTGCTCATGTGGG
TCACCTCGTTCAATCTCTGCACCTTTCCCACAAGGAGATGGGGGTGATGGATCGTCCATCTTAAAGATAC
AGAAACTGCCTTTTAAAGAGCAGAAGGGAAGGGAAGGGGTTGAGTCCTTCAGGACTAGCTAGAT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TCTGCTCATCCAAGTTGCAGGATG; Reverse Primer - name:unspecified, sequence:ATCTAGCTAGTCCTGAAGGACTC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9214 same embryo
 EMAGE:9212 same embryo
 EMAGE:9216 same embryo
 EMAGE:9213 same embryo
 EurExpress:euxassay_018112 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS