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EMAGE:9223

Tubb4a tubulin, beta 4A class IVA ( MGI:107848)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9223 EMAGE:9223 EMAGE:9223 EMAGE:9223 EMAGE:9223
"Pseudo-wholemount" of euxassay_018005. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018005_01 euxassay_018005_02 euxassay_018005_03 euxassay_018005_04
EMAGE:9223 EMAGE:9223 EMAGE:9223 EMAGE:9223 EMAGE:9223
euxassay_018005_05 euxassay_018005_06 euxassay_018005_07 euxassay_018005_08 euxassay_018005_09
EMAGE:9223 EMAGE:9223 EMAGE:9223 EMAGE:9223 EMAGE:9223
euxassay_018005_10 euxassay_018005_11 euxassay_018005_12 euxassay_018005_13 euxassay_018005_14
EMAGE:9223 EMAGE:9223 EMAGE:9223 EMAGE:9223 EMAGE:9223
euxassay_018005_15 euxassay_018005_16 euxassay_018005_17 euxassay_018005_18 euxassay_018005_19
EMAGE:9223 EMAGE:9223 EMAGE:9223 EMAGE:9223 EMAGE:9223
euxassay_018005_20 euxassay_018005_21 euxassay_018005_22 euxassay_018005_23 euxassay_018005_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9223Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9223_wholemount_strong.wlz
9223_wholemount_moderate.wlz
9223_wholemount_weak.wlz
9223_wholemount_possible.wlz
9223_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9223_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T51159
Entity Detected:Tubb4a, tubulin, beta 4A class IVA ( MGI:107848)
Sequence:sense strand is shown

>T51159
CATGGACGAGATGGAGTTTACGGAAGCAGAGAGCAATATGAACGACCTGGTGTCCGAGTACCAGCAGTAC
CAGGATGCCACTGCTGAAGAGGGCGAGTTCGAAGAGGAGGCTGAAGAGGAGGTGGCTTAAGTCTCCTGCC
ATCACTCTGTCCCTGGGGCCCACCAGCAAAGCTTTGACCCTAAGCATCACACCCCTGCACCTAGTTGCCT
CATTCCCTAGGACCCCATGAGCATCTTCACCATGAGGGCCAAGCCCAGGTTGCTTCTATTTGCTTCACCT
TTAACTCCTAAACCCCACTGTCTCTCCAACCTGCCAGGGAAGGGCTCTTCTAGTTCCCATGAGCGCCCCT
CAACACATGTACACACACACACACACTCCACCTTCTTAGATCTTGAAAATCCTTTCCTTTATGCCCTGTC
CCTTCCCCAGCACTCCTGAACCATTCTCCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCTTAACA
TTTTCCCCAGCCTCCAAGTCCTCTCACCTTGCCTTACCTCTGACCTCACAGCTACCCACTCCTGACCCAG
CACCTTCACTCTGGCTCCCGTAAGTCCCCGTATGTTCCTGCCCTTGTGCCTTAGTTCAGTTCCTACCAGA
GAAGTGCTGAAGGACGAGGAAAAA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CATGGACGAGATGGAGTTT; Reverse Primer - name:unspecified, sequence:TTCCTCGTCCTTCAGCACTT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9222 same embryo
 EMAGE:9221 same embryo
 EMAGE:9220 same embryo
 EurExpress:euxassay_018005 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS