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EMAGE:9235

E2f5 E2F transcription factor 5 ( MGI:105091)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9235
euxassay_018032_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9235Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9235_wholemount_strong.wlz
9235_wholemount_moderate.wlz
9235_wholemount_weak.wlz
9235_wholemount_possible.wlz
9235_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9235_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50344
Entity Detected:E2f5, E2F transcription factor 5 ( MGI:105091)
Sequence:sense strand is shown

>T50344
ATCAAAGAACAGTATCCAGTGGAAGGGTGTAGGTGCTGGCTGTAATACTAAAGAAGTTATCGATAGATTA
AGGTGTCTTAAAGCTGAAATTGAAGATCTCGAATTGAAGGAAAGAGAACTTGACCAGCAGAAGTTGTGGC
TACAGCAAAGCATCAAAAATGTGATGGAAGACTCCATTAATAACAGATTTTCTTATGTAACTCACGAAGA
CATCTGCAATTGCTTTCATGGTGATACACTGTTGGCCATTCAGGCACCTTCTGGTACACAGCTGGAAGTA
CCTATTCCAGAAATGGGACAGAATGGACAAAAGAAATACCAGATAAATCTGAAGAGTCACTCAGGGCCTA
TCCATGTGCTACTTATAAATAAAGAGTCCAGTTCATCTAAGCCAGTGGTTTTTCCTGTTCCCCCACCTGA
TGACCTCACACAGCCTTCCTCCCAGTCCTCAACTTCAGTGACTCCACAGAAATCCACCATGGCTGCTCAA
AACCTGCCTGAGCAGCATGTTTCCGAAAGAAGCCAGACTTTCCAGCAGACACCAGCTGCAGAAGTATCTT
CAGGATCTATTAGTGGAGACATCATTGATGAACTGATGTCTTCTGATGTGTTTCCTCTTTTACGGCTTTC
TCCTACCCCAGCAGATGACTACAACTTTAATTTAGATGATAATGAAGGAGTTTGTGATCTGTTTGATGTT
CAGATACTAAATTATTAGATTCCATGGAAACTTGGGACTATTATCTACCTCTATAACATTTTAGAATTCT
TTAATAACCTAAGTATTTAAAATTATGAATGTAACACCTTTTTAGTTCACTGATTCTGAAGTGTTCTTCC
CTAACATTTTATTTTTTA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TGTGGCTACAGCAAAGCATC; Reverse Primer - name:unspecified, sequence:GCCTGCTGTAACTGCCTCTC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9239 same embryo
 EMAGE:9238 same embryo
 EMAGE:9236 same embryo
 EMAGE:9237 same embryo
 EMAGE:9234 same embryo
 EurExpress:euxassay_018032 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS