Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:924

Pax2 paired box gene 2 ( MGI:97486)
TS15 (9.7 dpc)
in situ hybridisation

Data Images
EMAGE:924 EMAGE:924
Figure 3C of Martinez-Barbera et al., 2001 [PMID:11731459] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 3A' of Martinez-Barbera et al., 2001 [PMID:11731459] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
A' is a brightfield image of a closely parallel section of the darkfield image C. Image annotations: hb - hindbrain; mb - midbrain; fb - forebrain.
Expression Pattern Description
Spatial Annotation:
EMAGE:924EMAGE:924Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
924_voxel_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:924_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future brain
detected detected
regionalThe authors do not discuss this specimen in detail
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1098114
Entity Detected:Pax2, paired box gene 2 ( MGI:97486)
Notes:Probe used in this study by Martinez-Barbera et al., 2001 [PMID:11731459] is referenced as being described by Acampora et al., 1997 [PMID:9342056] who in turn refer to Avantaggiato et al., 1996 [PMID:8626038] who describe the probe as corresponding to an "RT-PCR amplified product spanning the region between amino acids 325 and 518". Pax2 protein (GenBank ID P32114) is 415 aa.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:C57BL/6 x DBA/2
Age:9.7 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
General Information
Authors:Martinez-Barbera et al., 2001 [PMID:11731459] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11731459] Martinez-Barbera JP, Signore M, Boyl PP, Puelles E, Acampora D, Gogoi R, Schubert F, Lumsden A, Simeone A 2001 Regionalisation of anterior neuroectoderm and its competence in responding to forebrain and midbrain inducing activities depend on mutual antagonism between OTX2 and GBX2. Development (128):4789-800
 [ PMID:9342056] Acampora D, Avantaggiato V, Tuorto F, Simeone A 1997 Genetic control of brain morphogenesis through Otx gene dosage requirement. Development (124):3639-50
 [ doi:10.1006/dbio.1996.0120] [ PMID:8626038] Avantaggiato V, Acampora D, Tuorto F, Simeone A 1996 Retinoic acid induces stage-specific repatterning of the rostral central nervous system. Dev Biol (175):347-57
Links:MGI:2155821 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI