EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:9257

Foxd1 forkhead box D1 ( MGI:1347463)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017989. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017989_01	euxassay_017989_02	euxassay_017989_03	euxassay_017989_04

euxassay_017989_05	euxassay_017989_06	euxassay_017989_07	euxassay_017989_08	euxassay_017989_09

euxassay_017989_10	euxassay_017989_11	euxassay_017989_12	euxassay_017989_13	euxassay_017989_14

euxassay_017989_15	euxassay_017989_16	euxassay_017989_17	euxassay_017989_18	euxassay_017989_19

euxassay_017989_20	euxassay_017989_21	euxassay_017989_22	euxassay_017989_23	euxassay_017989_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9257_wholemount_strong.wlz

9257_wholemount_moderate.wlz

9257_wholemount_weak.wlz

9257_wholemount_possible.wlz

9257_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9257_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50404
Entity Detected:	Foxd1, forkhead box D1 ( MGI:1347463)
Sequence:	sense strand is shown >T50404 CTTCTCCATCGAGAGCCTCATCGGGCGCACTCGGGGTCCCGCGGCCGCCGGGGCGCACGTCTCGTCCGGG GCCGCCAGCGGGACCGCCCCGGGACCCGGGGGCGGGGGTTGCGCAGTACAGGCGGCTGCTGGTCCCGCCG TCGCGCTCACCCGCTCGCTCGTGGTCGCCGCCGCCGCGGCCGCCTCCTCCGTGTCCTCGTCCGCCGCGCT GGGGACTCTGCACCAAGGGACAGCGCTGTCCAGTGTGGAGAACTTTACTGCTAGGATTTCCAATTGTTAG GAACGTCGTTAGCGCGCGGGAGAGCGAAGGTAGGACTCCCGGCTTCTTTCTCCGGATGGGGGGGTTGGTT TCGTTCGCCCCTCCCGGTCCTCGGAGACCCCGCGCCCCCCGTTTTCGCCGCTTCGGATTCTTGGACCAGA CTGTGTTGGGCGACAGCTGGGGCGCCGCGCAGTTTAGCTCAGAGGGTCCATCTATTTATGCAAAATCGCC CTATGCTGCAACCCTGACTTGGGGTGGGAAGGAGGGGAGTCGCTCTGTCTTGGCACTAGGAATTTCCTTG ACTTTTGACAAATTGAGAAAAAACAAAACAAAACAAGCAAAATCATCAAAACTAAGCCCTTTTTGAGGTG TAGAGATTCACAGGTCCAGCGTTTTAAAAAATCAGTAATGTTTAAATGCAGCTTATAGAAAACCAGTAAA AGTCTCCAAGAAATGCCTCTACTTGTTCACACTTGTTTGGTAGACTTTTTTCATGGAAAGAAAAAAAATT AACATGTTTACACAAGAAATAAGTCGAAATTTACCATTTCCTATTTTTAACCTGTGTTTTGTATCATAAT GGACATGCGGAATTTTT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CTTCTCCATCGAGAGCCTCA; Reverse Primer - name:unspecified, sequence:AAAAATTCCGCATGTCCATT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9259 same embryo
	EMAGE:9261 same embryo
	EMAGE:9260 same embryo
	EMAGE:9258 same embryo
	EMAGE:9262 same embryo
	EurExpress:euxassay_017989 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source