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EMAGE:926

Pax2 paired box gene 2 ( MGI:97486)
TS15 (9.5-10.0 dpc)
in situ hybridisation

Data Images
EMAGE:926 EMAGE:926
Fig 1B Martinez-Morales et al 2001 [PMID:11493524] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 1E Martinez-Morales et al 2001 [PMID:11493524] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Both are examples of a TS15 sample - (B) is 9.5dpc and (E) is 10.0dpc. Image B has been used for the raw data in this EMAGE entry. Image annotations: ov - optic vesicle.
Expression Pattern Description
Spatial Annotation:
EMAGE:926EMAGE:926Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
926_voxel_strong_3D_1.wlz
926_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:926_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
optic vesicle
detected detected
regionalExpression is in the ventral half
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1098114
Entity Detected:Pax2, paired box gene 2 ( MGI:97486)
Notes:Probe used in this study by Martinez-Morales et al 2001 [PMID:11493524] is referenced as being described by Acampora et al., 1997 [PMID:9342056] who in turn refer to Avantaggiato et al., 1996 [PMID:8626038] who describe the probe as corresponding to an "RT-PCR amplified product spanning the region between amino acids 325 and 518". Pax2 protein (GenBank ID P32114) is 415 aa.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:BALB/c
Age:9.5-10.0 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Staining procedure:autoradiography
General Information
Authors:Martinez-Morales et al 2001 [PMID:11493524] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1996.0120] [ PMID:8626038] Avantaggiato V, Acampora D, Tuorto F, Simeone A 1996 Retinoic acid induces stage-specific repatterning of the rostral central nervous system. Dev Biol (175):347-57
 [ PMID:9342056] Acampora D, Avantaggiato V, Tuorto F, Simeone A 1997 Genetic control of brain morphogenesis through Otx gene dosage requirement. Development (124):3639-50
 [ PMID:11493524] Martinez-Morales JR, Signore M, Acampora D, Simeone A, Bovolenta P 2001 Otx genes are required for tissue specification in the developing eye. Development (128):2019-30
Links:MGI:2137333 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI