EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:927

Aldh1a2 aldehyde dehydrogenase family 1, subfamily A2 ( MGI:107928)
TS11 (EHF Downs & Davies)
in situ hybridisation

Data Images


Figure 5A of Hochgreb et al., 2003 [PMID:13129847] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.	Figure 5B of Hochgreb et al., 2003 [PMID:13129847] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.	Figure 5C of Hochgreb et al., 2003 [PMID:13129847] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.	Figure 5D of Hochgreb et al., 2003 [PMID:13129847] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.	Figure 5E of Hochgreb et al., 2003 [PMID:13129847] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Aldh1a2 expression is shown in orange, while Tbx5 expression is shown in purple. Image annotations used: LB, late bud; EHF, early head fold. The white arrow in E indicates RALDH2 expression penetrating the cardiac field and overlapping posterior cardiac precursors. All of these embryos are TS11 but only the Aldh1a2 expression domain from embryo D was spatially mapped in this EMAGE entry as it is the best match to the EMAP TS11 embryo model (#1) .

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

927_wholemount_strong_3D_1.wlz

927_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:927_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo mesoderm

detected

regional

Fig 5A - Expression was detected in mesoderm posterior to the node. Fig 5B - Anterior expansion of expression was detected in the lateral mesoderm. Fig 5C - Anterior expansion of expression was detected in the lateral mesoderm. Fig 5D - Anterior expansion of expression was detected in the lateral mesoderm and had contacted the posterior-most cardiac precursors. Fig 5E - Anterior expansion of expression was detected in the lateral mesoderm and overlapped the posterior-most cardiac precursors.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:2684645
Entity Detected:	Aldh1a2, aldehyde dehydrogenase family 1, subfamily A2 ( MGI:107928)
Notes:	The Aldh1a2 (Raldh2) probe used in this study by Hochgreb et al., 2003 [PMID:13129847] was originally described in Zhao et al., 1996 [PMID:8797830] and is a "161-base sequence from the 3' untranslated region of RALDH-2 in pBluescript KS". Exact end points are not defined.
Chemistry:	RNA
Strand:	antisense

Specimen

Organism:	mouse
Strain:	FVB/N
Age:	EHF Downs & Davies
Theiler Stage:	TS11
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:

4% paraformaldehyde

General Information

Authors:	Hochgreb et al., 2003 [PMID:13129847] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1242/dev.00750] [ PMID:13129847] Hochgreb T, Linhares VL, Menezes DC, Sampaio AC, Yan CY, Cardoso WV, Rosenthal N, Xavier-Neto J 2003 A caudorostral wave of RALDH2 conveys anteroposterior information to the cardiac field. Development (130):5363-74
	[ doi:10.1111/j.1432-1033.1996.0015h.x] [ PMID:8797830] Zhao D, McCaffery P, Ivins KJ, Neve RL, Hogan P, Chin WW, Drager UC 1996 Molecular identification of a major retinoic-acid-synthesizing enzyme, a retinaldehyde-specific dehydrogenase. Eur J Biochem (240):15-22
Links:	MGI:2684665 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source