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EMAGE:9347

Nup62 nucleoporin 62 ( MGI:1351500)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9347 EMAGE:9347 EMAGE:9347 EMAGE:9347 EMAGE:9347
"Pseudo-wholemount" of euxassay_018451. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018451_01 euxassay_018451_02 euxassay_018451_03 euxassay_018451_04
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euxassay_018451_05 euxassay_018451_06 euxassay_018451_07 euxassay_018451_08 euxassay_018451_09
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euxassay_018451_10 euxassay_018451_11 euxassay_018451_12 euxassay_018451_13 euxassay_018451_14
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euxassay_018451_15 euxassay_018451_16 euxassay_018451_17 euxassay_018451_18 euxassay_018451_19
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euxassay_018451_20 euxassay_018451_21 euxassay_018451_22 euxassay_018451_23 euxassay_018451_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9347Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9347_wholemount_strong.wlz
9347_wholemount_moderate.wlz
9347_wholemount_weak.wlz
9347_wholemount_possible.wlz
9347_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9347_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50769
Entity Detected:Nup62, nucleoporin 62 ( MGI:1351500)
Sequence:sense strand is shown

>T50769
ATAGTCCTGCAGGTTTAAACGAATTCGCCCTTGCAGAGGGTCTGTGAATTAGCGCGCAGGCGCAACGGCG
CGCGAGCCGACCTTAAAAGCCCCCAAATTCTCCGGAAGCACGCCCTCCTCCTCCCCGCCCCTCGCAACTC
GAGCCTAGTGCGCTTGCTCAGCGGATGCGCCGGCCGCCAGCTTCCCTCTTCTGCGCCTGCGCGACCGTGG
TTCCTCACTTCGTCTCCCCCGCCCCCACCACGAGGCTGACCCGTGAAGCCCCGCGAGCTCTGAAAGGGCG
GGAAAGGCAGTTGGCGAGGGAGGGGCGAGCGGTTACTCACTCCATGGCGGCGAAAAGGCGAGGAGGCAAC
GGCGGCGGCGGCCTCGGCTGAAGAGACGGTGTGAGCGGAGAGTGAAGGCGCGAAACCTCCCCAAGGTTGC
AGACGCTGACGGATTTGCCCCAGAGTCGAAGTAGCTGCCACCTGAAGATTCCACAACCATGAGTGGATTT
AACTTTGGAGGCACCGGGGCTCCTGCTGGCGGCTTTACATTTGGGACCGCAAAGACTGCGACCACCACAC
CTGCCACTGGCTTTTCCTTCTCTGCTTCTGGCACCGGCACCGGAGGGTTTAATTTTGGGACTCCCTCCCA
GCCAGCTGCGACCACCCCTTCCACCAGCCTCTTCTCACTCACCACACAGAAGGGCGAATTCGCGGCCCGC
TAAATTCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGAC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9345 same embryo
 EMAGE:9346 same embryo
 EMAGE:9348 same embryo
 EurExpress:euxassay_018451 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS