EMAGE mouse gene expression spatial database

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EMAGE:9361

Fzd8 frizzled homolog 8 (Drosophila) ( MGI:108460)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018287. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018287_01	euxassay_018287_02	euxassay_018287_03	euxassay_018287_04

euxassay_018287_05	euxassay_018287_06	euxassay_018287_07	euxassay_018287_08	euxassay_018287_09

euxassay_018287_10	euxassay_018287_11	euxassay_018287_12	euxassay_018287_13	euxassay_018287_14

euxassay_018287_15	euxassay_018287_16	euxassay_018287_17	euxassay_018287_18	euxassay_018287_19

euxassay_018287_20	euxassay_018287_21	euxassay_018287_22	euxassay_018287_23	euxassay_018287_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9361_wholemount_strong.wlz

9361_wholemount_moderate.wlz

9361_wholemount_weak.wlz

9361_wholemount_possible.wlz

9361_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9361_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50424
Entity Detected:	Fzd8, frizzled homolog 8 (Drosophila) ( MGI:108460)
Sequence:	sense strand is shown >T50424 CTCAAGTTCTTTCTGTGTAGCATGTACACGCCCATCTGCCTGGAGGACTACAAGAAGCCTCTGCCGCCTT GTCGCTCTGTGTGTGAACGCGCCAAGGCCGGCTGCGCGCCGCTCATGCGCCAGTACGGCTTTGCTTGGCC TGACCGCATGCGCTGCGATCGGTTGCCGGAGCAGGGCAACCCGGACACTCTGTGCATGGACTACAACCGC ACCGACCTCACCACGGCCGCGCCCAGCCCACCGCGCCGCCTGCCTCCGCCGCCTCCTCCCGGCGAGCAGC CGCCCTCTGGCAGCGGCCACAGCCGCCCGCCAGGGGCCAGGCCCCCACATCGTGGCGGCAGCAGTAGGGG CAGCGGGGACGCGGCGGCTGCGCCCCCTTCGCGCGGCGGGAAGGCGAGGCCCCCTGGTGGCGGCGCTGCT CCCTGCGAGCCGGGGTGCCAGTGCCGCGCGCCCATGGTGAGCGTGTCCAGCGAACGCCACCCGCTCTACA ACCGCGTCAAGACCGGCCAGATCGCCAACTGTGCGCTGCCCTGCCACAACCCCTTCTTTAGCCAGGATGA GCGCGCCTTCACCGTCTTCTGGATCGGCCTGTGGTCGGTGCTCTGCTTCGTCTCCACCTTCGCCACTGTC TCTACCTTCCTCATCGATATGGAGCGCTTTAAGTACCCGGAACGGCCCATCATATTCCTCTCCGCCTGTT ACCTCTTCGTGTCTGTCGGGTACCTGGTGCGCCTGGTGGCAGGACATGAGAAAGTGGCCTGCAGCGGCGG CGCTCCGGGTGCTGGCGGAGCTGGGGGTGCGGGCGGCGCGGCGGCGGCTGGCGCAGGGGCAGCGGGAGCG GGGGCGAGCAGCCCGGGCGCGCGCGGCGAGTACGAGGAGCTGGGCGCAGTTGAGCAGCATGTTCGCTATG AGACCACTGGCCCCGCGCTGTGCACGGTGGTCTTTCTCCTTGTCTACTTTTTTGGCATGGCCAGCTCCAT CTGGTGGGTAATCCTGTCGCTCACGTGGTTCTTGGCAGCTGGCATGAAGTGGGGTAACGAGGCCATAGCA GGCTACTCGCAGTACTTCCACCTGGCCGCGTGGCTTGTGCCCAGCGTCAAGTCCATCGCGGTGCTGGCGC TCAGCTCCGTAGACGGCGACCCGGTGGCGGGCATCTGCTACGTGGGCAACCAGAGCCTTGACAACCTACG CGGCTTTGTGCTGGCGCCACTGGTTATCTACCTCTTCATTGGGACTATGTTTCTGTTAGCTGGCTTCGTG TCGCTGTTCCGAATCCGTTCAGTCATCAAGCAGCAAGGAGGTCCAACTAAGACACACAAGCTAGAAAAAC TCATGATCCGCTTGGGCCTCTTCACCGTGCTCTACACGGTGCCCGCTGCCGTCGTTGTCGCCTGCCTTTT CTATGAGCAGCACAACCGACCGCGCTGGGAGGCCACGCACAACTGCCCATGCCTTCGGGACCTGCAACCG GACCAGGCTCGCAGGCCCGATTACGCGGTCTTCATGCTCAAGTACTTCATGTGCCTAGTAGTGGGCATCA CATCGGGCGTGTGGGTCTGGTCCGGCAAGACTCTGGAGTCCTGGCGCGCGTTGTGCACTAGGTGCTGCTG GGCCAGCAAGGGCGCTGCAGTAGGCGCGGGCGCTGGAGGCAGCGGCCCTGGGGGCAGTGGACCCGGGCCC GGCGGAGGTGGGGGACACGGCGGAGGCGGGGGATCCCTCTACAGCGACGTCAGTACCGGCCTGACGTGGC GGTCTGGCACGGCCAGCTCTGTATCTTACCCTAAGCAAATGCCATTGTCCCAGGTCTGAACCCTACGTGG ATGCCCAGAAGGGGCGGAGAGGAGTGGGGGATGGGGAACCCGTGGGCGGCGAAGGGACCCCAGACCGGCC AGGGTTCCCACCCCTTCCCAGTGTTGACTGCTATTAGCATGACAATGAACTCTTAATGGTATCCATTAGC AGCGGGGACTTAAATGACTCCCTTAGAACAAAGTACCTGG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9365 same embryo
	EMAGE:9364 same embryo
	EMAGE:9363 same embryo
	EMAGE:9362 same embryo
	EurExpress:euxassay_018287 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source