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EMAGE:9372

Nkx2-4 NK2 transcription factor related, locus 4 (Drosophila) ( MGI:97349)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9372 EMAGE:9372 EMAGE:9372 EMAGE:9372 EMAGE:9372
"Pseudo-wholemount" of euxassay_018294. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018294_01 euxassay_018294_02 euxassay_018294_03 euxassay_018294_04
EMAGE:9372 EMAGE:9372 EMAGE:9372 EMAGE:9372 EMAGE:9372
euxassay_018294_05 euxassay_018294_06 euxassay_018294_07 euxassay_018294_08 euxassay_018294_09
EMAGE:9372 EMAGE:9372 EMAGE:9372 EMAGE:9372 EMAGE:9372
euxassay_018294_10 euxassay_018294_11 euxassay_018294_12 euxassay_018294_13 euxassay_018294_14
EMAGE:9372 EMAGE:9372 EMAGE:9372 EMAGE:9372 EMAGE:9372
euxassay_018294_15 euxassay_018294_16 euxassay_018294_17 euxassay_018294_18 euxassay_018294_19
EMAGE:9372 EMAGE:9372 EMAGE:9372 EMAGE:9372 EMAGE:9372
euxassay_018294_20 euxassay_018294_21 euxassay_018294_22 euxassay_018294_23 euxassay_018294_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9372Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9372_wholemount_strong.wlz
9372_wholemount_moderate.wlz
9372_wholemount_weak.wlz
9372_wholemount_possible.wlz
9372_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9372_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50739
Entity Detected:Nkx2-4, NK2 transcription factor related, locus 4 (Drosophila) ( MGI:97349)
Sequence:sense strand is shown

>T50739
CAGAACCATCGCTACAAGATGAAGAGGCAGGCCAAGGACAAGGCGGCGCAGCAGCTGCAACAGGAGGGCG
GCCTGGGCCCCCCGCCGCCTCCGCCGCCGCCGTCGCCGCGCCGCGTGGCGGTACCGGTGCTGGTGAAGGA
CGGCAAGCCGTGCCAGAACGGCGCGGGCACCCCGACGCCTGGCCAGGGAGGCCAGCAGCCGCAAGCCCCG
ACGCCCGCGCCAGAGCTCGAGGAGCTGTCGCCCAGCCCGCCCGCTCTGCATGGTCCCGGGGGCGGCTTAG
CGGCCCTGGACGCGGCCACCGGGGACTACGGCGGAGGCGTGCTCGGCGCCAACCTGCTCTATGGCAGAAC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CAGAACCATCGCTACAAGATGA; Reverse Primer - name:unspecified, sequence:TTCTGCCATAGAGCAGGTTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9371 same embryo
 EMAGE:9373 same embryo
 EurExpress:euxassay_018294 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS