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EMAGE:94

Gbx2 gastrulation brain homeobox 2 ( MGI:95668)
TS11
in situ hybridisation

Data Images
EMAGE:94
Fig. 5B of Martinez-Barbera et al, 2001 [PMID:11731459] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The embryo below is described by Martinez-Barbera et al., as "headfold" stage.
Expression Pattern Description
Spatial Annotation:
EMAGE:94Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
94_wholemount_strong_3D_1.wlz
94_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:94_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
regionalThe expression is posterior
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2155172
Entity Detected:Gbx2, gastrulation brain homeobox 2 ( MGI:95668)
Sequence:sense strand is shown

>MGI:2155172
ACGGACTGCAGGACGGACAGACGGACGGACGCACGCACTGCTGGTAGCTGGGCCGAGACCTGCAGACGCG
AAGCGCGCGCGTTCCGACGGGCTAGCAGCCGGGAGCGGTTCCGGCGAACGCGAGGTCCCCGGCGCCCTCT
CCATCCCGGGCGCACGGCCTCATCGCGCTCGGTGGCTGCACCCGTGCAGGCCGGGTATGCTGCGGGTCCT
GCACTGAGGGGCGGCGGCGGCGTCTGCGCTCTGGGCACCGCTCAGCCCCTGCCCGGAGTTCCCGGTGGTA
CTCCCAGCGTCCGGAGGGCTTCCAGGCGGCGGCGGGACACGGGGACTTTTCGTCTCTCCCTGGCTTCTCC
CGAACGCGTCTATGAGCGCAGCGTTCCCGCCGTCGCTGATGATGATGCAGCGCCCGCTGGGGAGTAGTAC
CGCCTTCAGCATAGACTCGCTGATCGGCAGCCCGCCGCAGCCCAGTCCCGGCCATTTCGTCTACACCGGC
TACCCCATGTTCATGCCCTACCGGCCGGTGGTGCTGCCGCCACCGCCGCCACCGCCTCCCGCGCTGCCCC
AGGCAGCGCTGCAGCCCGCTCTGCCGCCCGCGCACCCTCACCACCAGATCCCCAGCCTGCCCACCGGCTT
CTGCTCCAGCCTGGCGCAGGGCATGGCGCTCACCTCCACGCTCATGGCCACTCTGCCCGGCGGCTTCTCT
GCGTCGCCCCAGCACCAAGAGGCGGCGGCTGCCCGCAAGTTCGCTCCACAGCCACTGCCCGGAGGCGGCA
ACTTCGACAAAGCCGAGGCGCTCCAAGCGGATGCGGAAGACGGCAAAGCCTTCTTGGCCAAGGAGGG
nt 62 - nt 898 of NM_010262.1
Notes:The Gbx2 probe used in this study by Martinez-Barbera et al, 2001 [PMID:11731459] is described as a "PCR fragment 836bp long that included 361bp upstream and 475bp downstream the ATG".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6 x DBA/2
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Martinez-Barbera et al., 2001 [PMID:11731459] Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11731459] Martinez-Barbera JP, Signore M, Boyl PP, Puelles E, Acampora D, Gogoi R, Schubert F, Lumsden A, Simeone A 2001 Regionalisation of anterior neuroectoderm and its competence in responding to forebrain and midbrain inducing activities depend on mutual antagonism between OTX2 and GBX2. Development (128):4789-800
Links:MGI:2155561 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI