Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:941

Otx2 orthodenticle homolog 2 (Drosophila) ( MGI:97451)
TS08 (6.5 dpc)
in situ hybridisation

Data Images
EMAGE:941 EMAGE:941
Fig 3A Boyl et al, 2001 [PMID:11532921] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who own the copyright. Fig 3A' Boyl et al, 2001 [PMID:11532921] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who own the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
These two sections are described as sagittal by the authors and appear to be near adjacent sections from the same embryo. A is an in situ hybrisidation for Otx2 mRNA and A' is an antibody stain for the Otx2 protein. Image annotations: epi - epiblast, AVE - anterior visceral endoderm.
Expression Pattern Description
Spatial Annotation:
EMAGE:941EMAGE:941Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
941_voxel_strong_3D_1.wlz
941_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:941_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
epiblast
detected detected
primitive endoderm
detected detected
Authors state that expression is in the VE (visceral endoderm)
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2150046
Entity Detected:Otx2, orthodenticle homolog 2 (Drosophila) ( MGI:97451)
Notes:The probe used in this study by Boyl et al, 2001 [PMID:11532921] is described as such: "The Otx2 probe employed in in situ hybridisation experiments on gastrulating wild-type embryos was probe (a) in Fig. 1A". Exact probe end points are undefined but the probe covers the 3' end of exon 1.
Chemistry:RNA
Strand:antisense
Label:undefined
Specimen
Organism:mouse
Age:6.5 dpc
Theiler Stage:TS08
Mutations:none (wild-type)
Preparation:section
Procedures
Staining procedure:undefined
General Information
Authors:Boyl et al, 2001 [PMID:11532921] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11532921] Boyl PP, Signore M, Acampora D, Martinez-Barbera JP, Ilengo C, Annino A, Corte G, Simeone A 2001 Forebrain and midbrain development requires epiblast-restricted Otx2 translational control mediated by its 3' UTR. Development (128):2989-3000
Links:MGI:2150147 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI