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EMAGE:942

Myl2 myosin, light polypeptide 2, regulatory, cardiac, slow ( MGI:97272)
TS14 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:942
Fig 6F Roebroeck et al, 1998 [PMID:9811571] . Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: a,common atrial chamber; bc,bulbus cordis; en,endocardium; fg,foregut; v,common ventricular chamber.
Expression Pattern Description
Spatial Annotation:
EMAGE:942EMAGE:942Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
942_voxel_strong_3D_1.wlz
942_voxel_moderate_3D_1.wlz
942_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:942_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
heart
detected detected
regionalExpression is restricted to the ventricular and proximal outflow tracts
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1309867
Entity Detected:Myl2, myosin, light polypeptide 2, regulatory, cardiac, slow ( MGI:97272)
Notes:The Myl2 (Mlc2v) probe used in this study by Roebroeck et al, 1998 [PMID:9811571] is indicated as that described by Lyons et al, 1995 [PMID:7628699] ie: "transcribed with T7 polymerase from the BamHI linearized plasmid pcMLC2V (supplied by Kenneth Chien, University of Califormia, SanDiego), a 449-bp partial-length cDNA cloned into pBS". Probe end points are undefined.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:9.5 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount plus section of wholemount
Procedures
Embedding:paraffin
Staining procedure:undefined
General Information
Authors:Roebroeck et al, 1998 [PMID:9811571] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9811571] Roebroek AJ, Umans L, Pauli IG, Robertson EJ, van Leuven F, Van de Ven WJ, Constam DB 1998 Failure of ventral closure and axial rotation in embryos lacking the proprotein convertase Furin. Development (125):4863-76
 [ doi:10.1101/gad.9.13.1654] [ PMID:7628699] Lyons I, Parsons LM, Hartley L, Li R, Andrews JE, Robb L, Harvey RP 1995 Myogenic and morphogenetic defects in the heart tubes of murine embryos lacking the homeo box gene Nkx2-5. Genes Dev (9):1654-66
Links:MGI:1331444 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI