Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:9485

E2f3 E2F transcription factor 3 ( MGI:1096340)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9485 EMAGE:9485 EMAGE:9485 EMAGE:9485 EMAGE:9485
"Pseudo-wholemount" of euxassay_018075. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018075_01 euxassay_018075_02 euxassay_018075_03 euxassay_018075_04
EMAGE:9485 EMAGE:9485 EMAGE:9485 EMAGE:9485 EMAGE:9485
euxassay_018075_05 euxassay_018075_06 euxassay_018075_07 euxassay_018075_08 euxassay_018075_09
EMAGE:9485 EMAGE:9485 EMAGE:9485 EMAGE:9485 EMAGE:9485
euxassay_018075_10 euxassay_018075_11 euxassay_018075_12 euxassay_018075_13 euxassay_018075_14
EMAGE:9485 EMAGE:9485 EMAGE:9485 EMAGE:9485 EMAGE:9485
euxassay_018075_15 euxassay_018075_16 euxassay_018075_17 euxassay_018075_18 euxassay_018075_19
EMAGE:9485 EMAGE:9485 EMAGE:9485 EMAGE:9485 EMAGE:9485
euxassay_018075_20 euxassay_018075_21 euxassay_018075_22 euxassay_018075_23 euxassay_018075_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9485Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9485_wholemount_strong.wlz
9485_wholemount_moderate.wlz
9485_wholemount_weak.wlz
9485_wholemount_possible.wlz
9485_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9485_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50342
Entity Detected:E2f3, E2F transcription factor 3 ( MGI:1096340)
Sequence:sense strand is shown

>T50342
CCCTCCAGAAACGAGACTTGAAGTGCCTGACTCAATAGAGAGCCTACAAATCCATTTGGCAAGTACCCAA
GGGCCCATTGAGGTTTACTTGTGTCCAGAAGAGACGGAAACACACAGACCCATGAAAACAAATAACCAAG
ACCACAATGGGAATATCCCCAAGCCCACTTCCAAAGACTTGGCTTCTAACAACTCAGGACATAGTGACTG
CTCGGTTTCTACAGCAAACCTCTCTCCTCTGGCCTCCCCAGCCAACCTTTTACAGCAGACTGAGGACCAA
ATCCCGTCCAACCTTGAAGGACCTTTTGTGAACTTACTGCCTCCCCTGCTCCAAGAGGACTACCTGCTGA
GCCTGGGGGAGGAAGAGGGCATCAGTGACCTCTTCGATGCCTATGATTTGGAAAAGCTGCCTCTGGTGGA
GGACTTCATGTGTAGTTGATTGGGCTTTGTACAAACTGTCCTTATAAACCAATATTTTTTTATCACGGAA
TCAGAAACGTGTATCGCAGTGTTGTCCCTTCCTACCTTCTTCCTCCAAGAGTATCATGAAGTAAACTTCA
GAACAAAGCTGACATTTTGATGAATTAATTTTTTTTTTCTAAGCGCACAGTTGCAGGCTCCCTTGGGAAA
GCCCTGCCTGGTCCCAGGCTCCCAAATCTTCCTGATGAGTCAGCAAGTGAGAAGATGTGCAATCAGGTGT
CTCTCACCTGTCCTTCCCTCCCCTCCTTCCTCTCCTTTCCTTGCGGACTGGCTTGCTGTGCCTAACGGAT
GGGCTGTTGACTGGGTCCGGCCGCCTGGCCCGTTGGAAACAGCGCCCTCCCTTAACAGCAGTTCAAGCCG
TGCCTTCTCTTGCAGAATGCATGTCTTTGTGAGTCTGCTACCACGGAATGGAAT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CCCTCCAGAAACGAGACTTG; Reverse Primer - name:unspecified, sequence:ATTCCATTCCGTGGTAGCAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9483 same embryo
 EMAGE:9482 same embryo
 EMAGE:9486 same embryo
 EMAGE:9484 same embryo
 EurExpress:euxassay_018075 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS