Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:950

Olig2 oligodendrocyte transcription factor 2 ( MGI:1355331)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:950
Fig 1C of Nery et al., 2001 [PMID:11171336] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:950Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
950_wholemount_moderate_3D_1.wlz
950_wholemount_weak_3D_1.wlz
950_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:950_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
telencephalon
weak weak
regionalExpression is detected in the ventral telencephalon in the medial ganglionic eminence
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2148197
Entity Detected:Olig2, oligodendrocyte transcription factor 2 ( MGI:1355331)
Notes:The Olig2 (Olg-2) probe used in this study by Nery et al., 2001 [PMID:11171336] is defined as that described by Lu et al, 2000 [PMID:10719888] ie: "Antisense riboprobe for Olg-2 was generated by linearization of the plasmid pRBRAH16 (ATCC) with EcoRI and subsequent incubation with T3 RNA polymerase".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Nery et al., 2001 [PMID:11171336] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11171336] Nery S, Wichterle H, Fishell G 2001 Sonic hedgehog contributes to oligodendrocyte specification in the mammalian forebrain. Development (128):527-40
 [ doi:10.1016/S0896-6273(00)80897-1] [ PMID:10719888] Lu QR, Yuk D, Alberta JA, Zhu Z, Pawlitzky I, Chan J, McMahon AP, Stiles CD, Rowitch DH 2000 Sonic hedgehog--regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system. Neuron (25):317-29
Links:MGI:1930442 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI