Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:9500

Sox5 SRY-box containing gene 5 ( MGI:98367)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9500 EMAGE:9500 EMAGE:9500 EMAGE:9500 EMAGE:9500
"Pseudo-wholemount" of euxassay_018119. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018119_01 euxassay_018119_02 euxassay_018119_03 euxassay_018119_04
EMAGE:9500 EMAGE:9500 EMAGE:9500 EMAGE:9500 EMAGE:9500
euxassay_018119_05 euxassay_018119_06 euxassay_018119_07 euxassay_018119_08 euxassay_018119_09
EMAGE:9500 EMAGE:9500 EMAGE:9500 EMAGE:9500 EMAGE:9500
euxassay_018119_10 euxassay_018119_11 euxassay_018119_12 euxassay_018119_13 euxassay_018119_14
EMAGE:9500 EMAGE:9500 EMAGE:9500 EMAGE:9500 EMAGE:9500
euxassay_018119_15 euxassay_018119_16 euxassay_018119_17 euxassay_018119_18 euxassay_018119_19
EMAGE:9500 EMAGE:9500 EMAGE:9500 EMAGE:9500 EMAGE:9500
euxassay_018119_20 euxassay_018119_21 euxassay_018119_22 euxassay_018119_23 euxassay_018119_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9500Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9500_wholemount_strong.wlz
9500_wholemount_moderate.wlz
9500_wholemount_weak.wlz
9500_wholemount_possible.wlz
9500_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9500_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T51064
Entity Detected:Sox5, SRY-box containing gene 5 ( MGI:98367)
Sequence:sense strand is shown

>T51064
AACAAGCACAGATCCCCATCGCGACGGCCGGAGTTGTATACCCCAGCGCCATCGCCATGGCAGGAATGCC
GTCCCCTCACCTGCCCTCCGAGCACTCGAGCGTGTCCAGCAGCCCGGAGCCCGGGATGCCCGTGATCCAG
AGCACTTACGGCGCCAAGGGAGAGGAGCCCCACATCAAGGAAGAGATCCAGGCTGAGGACATCAACGGAG
AGATTTACGAGGAGTACGATGAGGAGGAGGAGGACCCGGATGTGGATTATGGGAGTGACAGCGAAAACCA
CATTGCGGGACAAGCCAACTGATAAGGGCCAACAGACTGTGGTGAGCCGAGGACTTGAAGAAGCCCTGTC
CGGTTCATCCTTCCCAGTGGCCAAGCACATTAACTCTCTCATACACTGACTGTTATTTTAACTGTTAGTC
TTATATAGTTGGGACATCAGCTGACAAATAGACCTCAGCCTCAAAAGGCTCGGAAAGAAAAGAAAGAAAG
AAAGAAAGAAAAAAAATTACAAGCAAAAACGTCATCAAGAAGAAGAGATCGAAATAAGCTATGGGTTAAA
CAGTGCCAGTAACTCAGCTGCTACACCCCAGCACTGAAGTCTCACCCGTCA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AACAAGCACAGATCCCATC; Reverse Primer - name:unspecified, sequence:TGACGGGTGAGACTTCAGTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9498 same embryo
 EMAGE:9501 same embryo
 EMAGE:9499 same embryo
 EurExpress:euxassay_018119 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS