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EMAGE:9505

Ctsl cathepsin L ( MGI:88564)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9505 EMAGE:9505 EMAGE:9505 EMAGE:9505 EMAGE:9505
"Pseudo-wholemount" of euxassay_018327. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018327_01 euxassay_018327_02 euxassay_018327_03 euxassay_018327_04
EMAGE:9505 EMAGE:9505 EMAGE:9505 EMAGE:9505 EMAGE:9505
euxassay_018327_05 euxassay_018327_06 euxassay_018327_07 euxassay_018327_08 euxassay_018327_09
EMAGE:9505 EMAGE:9505 EMAGE:9505 EMAGE:9505 EMAGE:9505
euxassay_018327_10 euxassay_018327_11 euxassay_018327_12 euxassay_018327_13 euxassay_018327_14
EMAGE:9505 EMAGE:9505 EMAGE:9505 EMAGE:9505 EMAGE:9505
euxassay_018327_15 euxassay_018327_16 euxassay_018327_17 euxassay_018327_18 euxassay_018327_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9505Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9505_wholemount_strong.wlz
9505_wholemount_moderate.wlz
9505_wholemount_weak.wlz
9505_wholemount_possible.wlz
9505_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9505_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50214
Entity Detected:Ctsl, cathepsin L ( MGI:88564)
Sequence:sense strand is shown

>T50214
TGATTTGAATTCTGTTGCCATTTTTACATGGGTTAAATGTTACCACTACTTAAAACTCCTGTTATAAACA
GCTTTATAATATTGAAAACTTAGTGCTTAATTCTGAGTCTGGAATATTTGTTTTATATAAAGGTTGTATA
AAACTTTCTTTACCTCTTAAAAATAAATTTTAGCTCAGTGTGTGTGTATGTGGGACTTCAGTGTGTAATG
TGTTGACAAATGTTCGGGACTAAATACTGATTCAGGGTGACATGGTACAGCAAGAAAAAGGTGGCAGGTG
CTCTGGAGCCTTCGGATTTCACCTCAGTGTCCCATCAGATTAGGTATGTGTTAGTGAATCTAGCTTGGGA
TAATTGTTTTCTTTTTGTGTGTGTACATGGTAAATGTGTGGAGTTCAGAGAACAACTCAGTTCCACAGTG
TTGGTCCTATCAGGTCCTAGCCATCAAAATAAGGTTGTCAGGCTGGGCCACAGGTGCCTTTTACTACTGA
GCTATCTTGCCAGCCCCACTAGTTTTTAAGTGACATAATTACGTATGTTTATACTATAATGTGTTTTTGA
TATGATGTGTATACTACAAAATGATCTGATAGGGCTAATAAGCTTACCTCAAATATTTAATTACTTTGTT
GAACTTTTCTAGCTGTTTTTAAAATACACAGTATATTATTGTTGGAAACTGTCCTGTTATGTAGACCAGG
CTGGC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_018327 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS