EMAGE mouse gene expression spatial database

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EMAGE:9515

Prkag2 protein kinase, AMP-activated, gamma 2 non-catalytic subunit ( MGI:1336153)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018355. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018355_01	euxassay_018355_02	euxassay_018355_03	euxassay_018355_04

euxassay_018355_05	euxassay_018355_06	euxassay_018355_07	euxassay_018355_08	euxassay_018355_09

euxassay_018355_10	euxassay_018355_11	euxassay_018355_12	euxassay_018355_13	euxassay_018355_14

euxassay_018355_15	euxassay_018355_16	euxassay_018355_17	euxassay_018355_18	euxassay_018355_19

euxassay_018355_20	euxassay_018355_21	euxassay_018355_22	euxassay_018355_23	euxassay_018355_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9515_wholemount_strong.wlz

9515_wholemount_moderate.wlz

9515_wholemount_weak.wlz

9515_wholemount_possible.wlz

9515_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9515_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50872
Entity Detected:	Prkag2, protein kinase, AMP-activated, gamma 2 non-catalytic subunit ( MGI:1336153)
Sequence:	sense strand is shown >T50872 CCGCGGAAGCCGGCATGCTGGAGAAGCTGGAGTTCCAGGAGGAAGAAGACTCAGAAAGTGGCTTTTATAT GCGATTCATGAGGTCACACAAGTGTTATGACATCGTTCCAACCAGTTCAAAGCTTGTTGTCTTCGACACT ACGTTGCAAGTCAAAAAGGCCTTCTTTGCCTTGGTAGCCAACGGAGTCCGTGCAGCGCCGCTGTGGGAAA GTAAAAAGCAGAGCTTTGTAGGAATGCTCACGATTACAGATTTCATAAATATCCTACACAGATACTATAA ATCACCCATGGTACAGATTTATGAATTGGAAGAACATAAGATTGAAACGTGGAGGGAGTTGTACTTACAA GAAACCTTCAAGCCTTTGGTGAACATCTCTCCAGATGCGAGCCTCTTCGATGCTGTATACTCGTTGATCA AAAATAAAATCCACAGATTGCCAGTTATTGACCCTATCAGTGGGAACGCACTTTATATACTTACCCACAA AAGAATCCTCAAGTTCCTCCAGCTTTTTATGTCTGACATGCCAAAGCCTGCCTTCATGAAGCAGAACCTG GATGAGCTTGGAATCGGAACGTACCACAATATTGCCTTCATTCACCCAGACACTCCCATCATCAAAGCCT TGAACATCTTCGTGGAGAGACGGATATCCGCATTGCCTGTGGTGGATGAGTCAGGAAAAGTTGTAGATAT TTATTCCAAGTTTGATGTAATTAATCTTGCTGCTGAGAAAACGTACAATAACTTGGACATCACAGTGACC CAGGCCCTGCAGCACCGCTCGCAGTATTTTGAGGGTGTGGTGAAGTGCAGTAAGCTGGAAACACTGGAGA CCATCGTGGACAGGATAGTGAGAGCCGAGGTCCATCGGCTGGTTGTAGTGAATGAAGCAGATAGCATTGT GGGTATTATCTCCCTGTCAGACATCCTGCAAGCCCTGATCCTCACACCAGCAGGTGCCAAACAGAAGGAG ACAGAGACTGAATGACCGCCGTGAATGTAGACGCCCGCGCAGGAGAACTTGAACAAAGTCTCGGGGTCAC GGTTTGCCTCATGAACACTGGCTGCAATAGAAGAGAGTAAAATGGCTAAGAATGTATACCAGGGTTTAGC GATGGGTATTTTTCCAGTGATTTGTAAGTATAGGAAAAGATTTTATGTGCTTGAAGATTCAGGCTTGCGT TCAAAGACTGCCCTCAGACCTGTCTGAAGGATTTTCAATGCTGTATGTCATTAAAGTGCACTGTGTCCTG AAATAGTACTACTTTTCATTTCAAAGAAGTCACTGGGATGGAACAGGTGACAGAAAGTGAGTGAACGGCA CGTCCAGATCACGGTGCCTTATGTCCAAACGCAATATGTCACCGCCGCTGCCGCACGCCCCAAAGTGCTG CTGAAGAGACACCCAGCCCAAGTGTGAGCATCTTCAGACCTTTTACCCAGTCTCTTCCTTTTCACTTAAT TGGTTGAAAAGTTTTAATTTGAGTACAGGCAACTGAAAATGTGATGTGATGCCTCTAAGCGCTCTGCATC CGTCCTGTTCTGGCTGCCGTGTTAGCGAGGCCGGCTTGGCAACAGCGCTTTATTTATTTTTACTCTCATG CAGGTTTCAACCTACATCCTTTGGTGAGTAGACGTCACCATACCTACGAATAAAACCTTGATTGTTCTCT CAAGTGGAAAATCTCTGGAGATTTTAGTTTGGATCTGGAAAGGCTATGCCTTCCAAACAGCCACTGTAGG GTCTTCTAAACGTTTATGAAAACAGGCTGTGGTCGCAGGGAAGAAGTAACGGAAGACGTCTTTTTTTTTA TTGTGTGTTGAAATGTAAACTCATGATGTTTGTGTGACCGCTGACAAGACGCTTGTAACTTTTATTGTGA CACATAAAACAGTATTGTCATGCAGTTGAAGGGAAACTCTTTCCCCTCCCACACCCCACAACCCCCACAG CCCCCAGTGTCAGTGCTCTGAAAATGTTGACACTATATAT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9513 same embryo
	EMAGE:9514 same embryo
	EMAGE:9512 same embryo
	EurExpress:euxassay_018355 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source