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EMAGE:9541

Ghrh growth hormone releasing hormone ( MGI:95709)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9541 EMAGE:9541 EMAGE:9541 EMAGE:9541 EMAGE:9541
"Pseudo-wholemount" of euxassay_018420. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018420_01 euxassay_018420_02 euxassay_018420_03 euxassay_018420_04
EMAGE:9541 EMAGE:9541 EMAGE:9541 EMAGE:9541 EMAGE:9541
euxassay_018420_05 euxassay_018420_06 euxassay_018420_07 euxassay_018420_08 euxassay_018420_09
EMAGE:9541 EMAGE:9541 EMAGE:9541 EMAGE:9541 EMAGE:9541
euxassay_018420_10 euxassay_018420_11 euxassay_018420_12 euxassay_018420_13 euxassay_018420_14
EMAGE:9541 EMAGE:9541 EMAGE:9541 EMAGE:9541 EMAGE:9541
euxassay_018420_15 euxassay_018420_16 euxassay_018420_17 euxassay_018420_18 euxassay_018420_19
EMAGE:9541 EMAGE:9541 EMAGE:9541 EMAGE:9541 EMAGE:9541
euxassay_018420_20 euxassay_018420_21 euxassay_018420_22 euxassay_018420_23 euxassay_018420_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9541Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9541_wholemount_strong.wlz
9541_wholemount_moderate.wlz
9541_wholemount_weak.wlz
9541_wholemount_possible.wlz
9541_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9541_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50501
Entity Detected:Ghrh, growth hormone releasing hormone ( MGI:95709)
Sequence:sense strand is shown

>T50501
ACAGAGTCCCACCCAGGAGTGAAGGATGCTGCTCTGGGTGCTCTTTGTGATCCTCATCCTCACCAGTGGC
TCCCACTGCTCACTGCCCCCCTCACCTCCCTTCAGGATGCAGCGACACGTAGATGCCATCTTCACCACCA
ACTACAGGAAACTCCTGAGCCAGCTGTATGCCCGGAAAGTGATCCAGGACATCATGAACAAGCAAGGGGA
GAGGATCCAGGAACAAAGGGCCAGGCTCAGCCGCCAGGAAGACAGCATGTGGACAGAGGACAAGCAGATG
ACCCTGGAGAGCATCTTGCAGGGATTCCCAAGGATGAAGCCTTCAGCGGACGCTTGAGCCCCCCGAGCCC
CAAACACAACTGTACCCTGTTACTTCTGCTTCAGCTCTGACCTTTTCCGTCCTCTG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ACAGAGTCCCACCCAGGAGT; Reverse Primer - name:unspecified, sequence:CAGAGGACGGAAAAGGTCAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9540 same embryo
 EurExpress:euxassay_018420 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS