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EMAGE:958

Shh sonic hedgehog ( MGI:98297)
TS15 (22 Somite no.)
in situ hybridisation

Data Images
EMAGE:958
Figure 1B of Ybot-Gonzalez et al., 2002 [PMID:11973281] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:958Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
958_wholemount_strong_3D_1.wlz
958_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:958_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
notochord
detected detected
gradedThere is staining along the entire length of the notochord, but its intensity under the posterior neuropore varies according to developmental stage.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:23359
Entity Detected:Shh, sonic hedgehog ( MGI:98297)
Notes:The Shh probe used in this study by Ybot-Gonzalez et al., 2002 [PMID:11973281] was previously used by Greene et al., 1998 [PMID:9545534] and was previously described by Echelard et al., 1993 [PMID:7916661] as "either a 2.8 kb or 0.6 kb RNA transcript generated by T7 (2.8 kb) or T3 (0.6 kb) transcription of XbaI and HindIII digests of Bluescript (Stratagene) subclones of the original Shh cDNA inserts."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:22 Somite no.
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Ybot-Gonzalez et al., 2002 [PMID:11973281] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11973281] Ybot-Gonzalez P, Cogram P, Gerrelli D, Copp AJ 2002 Sonic hedgehog and the molecular regulation of mouse neural tube closure. Development (129):2507-17
 [ doi:10.1016/S0925-4773(98)00029-X] [ PMID:9545534] Greene ND, Gerrelli D, Van Straaten HW, Copp AJ 1998 Abnormalities of floor plate, notochord and somite differentiation in the loop-tail (Lp) mouse: a model of severe neural tube defects. Mech Dev (73):59-72
 [ doi:10.1016/0092-8674(93)90627-3] [ PMID:7916661] Echelard Y, Epstein DJ, St-Jacques B, Shen L, Mohler J, McMahon JA, McMahon AP 1993 Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity. Cell (75):1417-30
Links:MGI:2178345 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI