EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:9598

Dusp9 dual specificity phosphatase 9 ( MGI:2387107)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018924. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018924_01	euxassay_018924_02	euxassay_018924_03	euxassay_018924_04

euxassay_018924_05	euxassay_018924_06	euxassay_018924_07	euxassay_018924_08	euxassay_018924_09

euxassay_018924_10	euxassay_018924_11	euxassay_018924_12	euxassay_018924_13	euxassay_018924_14

euxassay_018924_15	euxassay_018924_16	euxassay_018924_17	euxassay_018924_18	euxassay_018924_19

euxassay_018924_20	euxassay_018924_21	euxassay_018924_22	euxassay_018924_23	euxassay_018924_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9598_wholemount_strong.wlz

9598_wholemount_moderate.wlz

9598_wholemount_weak.wlz

9598_wholemount_possible.wlz

9598_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9598_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

moderate

regional

moderate expression: see section 15 16 17

stomach

weak

regional

weak expression: see section 15 16 17 18 19 20 21

rectum

moderate

regional

moderate expression: see section 12 13

lower jaw incisor

weak

regional

weak expression: see section 10 11 12 14 15 16

upper jaw incisor

weak

regional

weak expression: see section 10 11 15 16

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

metanephros

weak

regional

weak expression: see section 06 07 08 09 10 16 17 18 19 20

lung

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50338
Entity Detected:	Dusp9, dual specificity phosphatase 9 ( MGI:2387107)
Sequence:	sense strand is shown >T50338 TATAAGCAGGGATGGGATGCAGCCATAAGCAGGGAGAGGGGAAAGGGGAAGGGGGGAGGGAAAGCGCAAT ACCTCATGTGGGGATGGTGGAGCAAGGCAGGAACAGAGTAGAAACCTGCCCCACCCACTCAAGGCCAGCC CCAGATTCTATTTCACGTGCCTACTGGTGGGCCCGGGCTTCGGGCCTGACCCTCACCGCTCTAGAGCGGG GCCATGCTCCAGAACCTGCCTCGTCTGTGACTGTTACTTTCTTTTTTCAGGGTGGGAGTGGGGCTTCCTA TCCAAGAGACCATTCCAGATTGCTGCCTGGGCCCTGGCCCTTCTTGCTGTTTCATTTTCAGGAAGAGTGT ATCATTCTTTGCCACAGGAACCTGCTCTTGGCTCCATACCTGTCCCCGAATGCCCACCTGTGGGAGCAGA CCTGGCCACTTTGACTCGTGTAGACTCCTTCCTGCTTCTCTCACTAGGGCTTAGACTTCTTGGAACTGTA GGGTGTGAACCCAGAGACACAATGGCTGCTCTCCGCATCCACAGGGACAGCCTCTCTGGGTGGCTTTTTG CTGGGCAGTGTCACATGGGAGGGCATGGGCCTGGGAGCCAATGGGCGAGATCCCAGCCACACATCTGGTG CCCTGACTGTTTCTTTCCTCCTCCCCCAGATGTCTTGACAGGATCACTGAGACTCTTTGTGACTGAGGGT GGTCAAACTGCCACCGGAGGAGATAGGGTCTCAGAGCTAGAGCTGAGGGGGAGGGAGGGAAAGATGAAGG CCTCAATTTTGCTACTGTGAGTCTCACACAGCCACTGTTGGTTTGGGGGGCAGGGAAGGGGCCAAGCTGC AGACACACATGGTCATTCATTGCAGTCCACA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TATAAGCAGGGATGGGATGC; Reverse Primer - name:unspecified, sequence:TGTGGACTGCAATGAATGAC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9600 same embryo
	EMAGE:9599 same embryo
	EMAGE:9601 same embryo
	EurExpress:euxassay_018924 same experiment
	MGI:4824422 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source