EMAGE mouse gene expression spatial database

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EMAGE:9614

4932438H23Rik RIKEN cDNA 4932438H23 gene ( MGI:1921637)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007195. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007195_01	euxassay_007195_02	euxassay_007195_03	euxassay_007195_04

euxassay_007195_05	euxassay_007195_06	euxassay_007195_07	euxassay_007195_08	euxassay_007195_09

euxassay_007195_10	euxassay_007195_11	euxassay_007195_12	euxassay_007195_13	euxassay_007195_14

euxassay_007195_15	euxassay_007195_16	euxassay_007195_17	euxassay_007195_18	euxassay_007195_19

euxassay_007195_20	euxassay_007195_21	euxassay_007195_22	euxassay_007195_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9614_wholemount_strong.wlz

9614_wholemount_moderate.wlz

9614_wholemount_weak.wlz

9614_wholemount_possible.wlz

9614_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9614_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 11 12 13 14 15 weak expression: see section 10 17 18

thalamus mantle layer

moderate

regional

moderate expression: see section 09 10 11 17 18 19 20 weak expression: see section 08

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 17 18 weak expression: see section 09 10 11 16

rest of cerebellum marginal layer

strong

regional

strong expression: see section 05 06 07 09 10 11 12 13 14 17 18 19 21 22 moderate expression: see section 08 15 weak expression: see section 20

midbrain mantle layer

moderate

regional

moderate expression: see section 12 13 14 15 16

heart left ventricle

strong

spotted

strong expression: see section 10 11 12 13

heart right ventricle

strong

spotted

strong expression: see section 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50002
Entity Detected:	4932438H23Rik, RIKEN cDNA 4932438H23 gene ( MGI:1921637)
Sequence:	sense strand is shown >T50002 ACAAAGGCTGGCAAACATGTATGTTCATCTCATTCTTAGATGTGGCTCTTTTCAACGGGGACTCATCTTT AAAGTCATACAGTATTGACAACATTTCTAGCCTCGCCAGTGACTTTCCTGACTTTTCTTACTTTAAAACG TCCCCAATGCCAAGCAACAGAAGCTATGTTGTCACAGTTATTTACTAGCATCCTGTGTCCCTCCACCAGG AACTCTAAAAAAGAAAAGAAAAAAAAAAGCATTTGAACAGGAATCTGGGAATAAGGTCGTAAGACAGTTG TCCCTGTTCCTTCTCTTCGCCTCCGTTTACAACAAAGCCACACCTAAGAGCAGCCAGCTCTCGGTTCCAC TTTCAATGCCAAGAGCAATTTGACTCCTGAACTTTGTCCCAAGGGCCAGCTAGAAGCCAGGTCTCTGCAA CTGGTAGCCTGTGGAGAGCTGTCAGGTGAGAAGGGGACAGAGTAGTGCACACCAGGGTCTACTTCATGGA GCTCAGTATAGTCATAGCCCCAAGGAAGGGACCTTCTTTCAGAATGCAGTTAACTCTTCCTTACCTGTGT GGGTGCATTCCAAGAGCCTGCGGACACCTGAATGAGTGAACAGTACTAACCCTATGCATACTGTATTTTC CGATAGAGACTTTTGACAAAATCTAACTTATAAATTAAGATCACTGGGGGGTTAAGGAAAAACTTTAACA TACTGTAAGAAAGTCGTGCAAACACGCTCGCTCACACCATTACACTGTGCTCGCGCTTCCCGTGACGACC GCTCTCTTTGGCTTAGAAACATTTCCAGCTCTGCTACTTTGTACATGATGGCTATTACGGAATAAAACAC AGGGTTGCTTTGTTTGTTTTGGTCAGTCTGACAACAGAGAGCAGTCACTGAGGGGCTGCTGGGTGGCAGT GTATTTAGGGTTTCTAGGACAGGCAAGCAGGCGACTCTGTCCCAGGTAGATCAGGCAGGAGCCCACTCTT TACCCTCCCCAGAACAGCTTGAAGTTTAAAACTTCTGATTTATAGATGAAACTGTATTTCAGGGACCACA GTGACCCTCTTCTTTCTTTTCCTTTTCATTTTGTGACATGGTCTGGAATTCCCATTCAAAAATGACACAT TTCTTAAGTTTGGGGACCTAAATTCCTCTAGACGGATATTTTCCAGATAGTTATGAAGAACAAATGCATT GAGAGACTGGAGTTTAAAAGTGTGTTTATGGACTTGGGAAGGTTGTGTGGGACCTTAACTCTCCCGGAAG GTGTCAGGGTTCCAAGAGAGGGGTGCACGAATGAAGACATTTGCCCAGACTGTTAGCCTCAAATTTACAC ACACAGCATTGTTGTGTGGGGAAGTCCTTTTCTGGTCTCTCTTCACTTTCACAGAGCAGTCACAGAGCAG ACCACAAGGCTCTTTGGCTCTTCCCTTTCCTCTCCTCTGCATCTGTTTAAGCATCTTTGTTCCCTCTATC CCTGATCTTATCACTTGCCCTCCCATCATCCCTTTCCCTCTTGCTCCTGAATTCAGAACTTAGATTACTG TCAGTACAATTGTTAAGCACCCGAGGGCTAGCTGGGGGGAATCTAACAATCACGTCAAACT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an T7 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using T7 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9613 same embryo
	EMAGE:9612 same embryo
	EurExpress:euxassay_007195 same experiment
	MGI:4822752 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source