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EMAGE:963

Pax6 paired box gene 6 ( MGI:97490)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:963
Figure 2B of Xu et al., 1999 [PMID:9847251] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: one star
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: lp - lens placode; ov - optic vesicle
Expression Pattern Description
Spatial Annotation:
EMAGE:963EMAGE:963Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
963_voxel_moderate_3D_1.wlz
963_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:963_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
lens placode
moderate moderate
optic vesicle
moderate moderate
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1328204
Entity Detected:Pax6, paired box gene 6 ( MGI:97490)
Sequence:sense strand is shown

>MGI:1328204
GTGCATTTGCATGTTGCGGAGTGATTAGTGGGTTTGAAAAGCGAACCGTGGCTCGGCCTCATTTCCCGCT
CTGGTTCAGGCGCAGGAGGAAGTGTTTTGCTGGAGGATGATGACAGAGGTCAGGCTTCGCTAATGGGCCA
GTGAGGAGCGGTGGAGGCGAGCCGGGCGCCGGCACACACACATTAACACACTTGAGCCATCA
nt 3884 - nt 4085 of AF098640.1
Notes:The Pax6 probe used in this study by Xu et al., 1999 [PMID:9847251] specifically detects a product transcribed from the P1 promoter and is described as: "201bp of exon 1 (from nucleotide position +1 to +201, as shown in Fig. 1D)." The GenBank accession number for the promoter is given as AF008211 (which has been subsequently replaced by AF098640).
Chemistry:DNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Xu et al., 1999 [PMID:9847251] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9847251] Xu PX, Zhang X, Heaney S, Yoon A, Michelson AM, Maas RL 1999 Regulation of Pax6 expression is conserved between mice and flies. Development (126):383-95
Links:MGI:1328242 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE