EMAGE mouse gene expression spatial database

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EMAGE:964

Gbx2 gastrulation brain homeobox 2 ( MGI:95668)
TS11 (7.75 dpc)
in situ hybridisation

Data Images


Figure 6C of Wassarman et al., 1997 [PMID:9247335] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity:
Find spatially similar expression patterns:

Notes:

Faint non-specific background staining is observed in the anterior region of the embryo (above top arrow).

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

3D context movie

View mapped 3D expression image

Download individual expression domains:

964_voxel_strong_3D_1.wlz

964_voxel_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:964_all_domains.zip

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

neural ectoderm

detected

regional

There is a stripe of Gbx2 expression in posterior neuroectoderm cells that expresses neither Hoxb1 nor Otx2

embryo

detected

regional

Expressed in all three germ layers in a domain similar to that of Hoxb1, but extending slightly further rostrally, abutting the posterior boundary of the Otx2 expression domain

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:26910
Entity Detected:	Gbx2, gastrulation brain homeobox 2 ( MGI:95668)
Sequence:	sense strand is shown >MGI:26910 GGCGCCCGCCAGAGACAAGTGCGCCGGCTTCGCGCGCTCCCCAGCCGCCCGTGGGAGACGACGGACTGCA GGACGGACAGACGGACGGACGCACGCACTGCTGGTAGCTGGGCCGAGACCTGCAGACGCGAAGCGCGCGC GTTCCGACGGGCTAGCAGCCGGGAGCGGTTCCGGCGAACGCGAGGTCCCCGGCGCCCTCTCCATCCCGGG CGCACGGCCTCATCGCGCTCGGTGGCTGCACCCGTGCAGGCCGGGTATGCTGCGGGTCCTGCACTGAGGG GCGGTGGTGGTGTCTGTGCTCTGGGCACCGCTCAGCCCCTGCCCGGAGTTCCCGGTGGTACTCCCAGCGT CCGGAGGGCTTCCAGGCGGCGGCGGGACACGGGGACTTTTCGTCTCTCCCTGGCTTCTCCCGAACGCGTC TATGAGCGCAGCGTTCCCGCCGTCGCTGATGATGATGCAGCGCCCGCTGGGGAGTAGTACCGCCTTCAGC ATAGACTCGCTGATCGGCAGCCCGCCGCAGCCCAGTCCCGGCCATTTCGTCTACACCGGCTACCCCATGT TCATGCCCTACCGGCCGGTGGTGCTGCCGCCACCGCCGCCACCGCCTCCCGCGCTGCCCCAGGCAGCGCT GCAGCCCGCTCTGCCGCCCGCGCACCCTCACCACCAGATCCCCAGCCTGCCCACCGGCTTCTGCTCCAGC CTGGCGCAGGGCATGGCGCTCACCTCCACGCTCATGGCCACTCTGCCCGGCGGCTTCTCTGCGTCGCCCC AGCACCAAGAGGCGGCGGCTGCCCGCAAGTTCGCTCCACAGCCACTGCCCGGAGGCGGCAACTTCGACAA AGCCGAGGCGCTCCAAGCGGATGCGGAAGACGGCAAAGCCTTCTTGGCCAAGGAGGGCTCGCTGCTCGCT TTCTCTGCGGCCGAAGCGGTGCAGGCGTCGCTCGTCGGGGCTGTCCGAGGGCAAGGGAAAGACGAGTCAA AGGTGGAAGATGACCCGAAGGGCAAGGAGGAGAGCTTCTCTCTGGAGAGCGATGTGGATTACAGCTCAGA TGACAATTTGCCTGGTCAGACTGCTCATAAGGAAGAAGACCCCGGCCACGCACTGGAGGAGACCCCGCAG AGCGGCGGTGCAGCAGGCAGCACCACGTCCACAGGCAAGAACCGGCGGCGGCGGACTGCCTTCACCAGCG AACAGCTGCTGGAGCTGGAGAAAGAATTCCACTGCAAAAAGTACCTCTCCCTGACCGAGCGCTCACAGAT CGCCCACGCCCTCAAACTCAGCGAGGTGCAAGTAAAAATCTGGTTCCAGAACCGCCGGGCCAAGTGGAAA CGTGTCAAGGCAGGCAACGCCAATTCCAAGACGGGGGAGCCCTCTCGGAACCCCAAGATTGTCGTCCCCA TCCCTGTTCACGTTAGCAGGTTCGCTATTCGAAGTCAACACCAGCAGCTGGAGCAGGCCCGACCCTGAGG GCCCAGCCAGGGTGGACACCACCATGGAGAGGCCCTAACACCTGAAGGAACCCGTGTCAGACTCCACTGT TTAAAGCAGAACTCAGAGGCACCTCCTAGATGTGGACATCCAAGCAAATTGAGAGTATATTCATTAGACG GGCTTAAAGGGGGCTACAGCCACACCAGAAGATACACTAAATATTTATTATACAACCCTACTTTGTACAT AGATCTCTATCTAGACTGGATCTCAGCTGCAGTGTTTTGAAAGGGATAGGATCAAATCTCTCTCCTTACA CTTTCCAGATGAAACAAAACCACCACTGTACTCTGTCGCCTTCAGCGACCACCTTCCCATACGTGCCCAA AGGTAAACAGGAGACACAAGGACAAACACTGCGGTTTAACCTTCTTATTGTTATTTTTTGTTTGTTTGTT TCTATTTTGGCTTTTTGAGTTGAGTGCAGAGCAGTCGGTTGATTTTGGAGCAATCTAGAACTCTGCTCTG CTCGTTTGCTTTGCCTGGCTGTCTTTCCTGGCCTTGTATATCCTATCTGAGCCATGTCGGAAGCACGTCT CCGTTGTGTTTAATTTATTTCAATGTAGCTTATTTTCATATAAGTTATGACATTTAAACAATCTCAGTCT TGTGAATAATAAAAAAAAAGGCGAGG
	nt 1 - nt 2126 of Z48800.1
Notes:	The probe used in this study by Wassarman et al., 1997 [PMID:9247335] is indicated as that described by Bouillet et al., 1995 [PMID:8601031] ie. "a 2.1kb Stra7/Gbx-2 cDNA". See Figure 1 therein and GenBank Z48800 for full sequence.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	7.75 dpc
Theiler Stage:	TS11
Mutations:	none (wild-type)
Preparation:	section

Procedures

Embedding:	paraffin
Staining procedure:	alkaline phosphatase + BMpurple

General Information

Authors:	Wassarman et al., 1997 [PMID:9247335] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:9247335] Wassarman KM, Lewandoski M, Campbell K, Joyner AL, Rubenstein JL, Martinez S, Martin GR 1997 Specification of the anterior hindbrain and establishment of a normal mid/hindbrain organizer is dependent on Gbx2 gene function. Development (124):2923-34
	[ doi:10.1002/aja.1002040404] [ PMID:8601031] Bouillet P, Chazaud C, Oulad-Abdelghani M, Dolle P, Chambon P 1995 Sequence and expression pattern of the Stra7 (Gbx-2) homeobox-containing gene induced by retinoic acid in P19 embryonal carcinoma cells. Dev Dyn (204):372-82
Links:	MGI:1331440 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source