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EMAGE:9646

Aco2 aconitase 2, mitochondrial ( MGI:87880)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9646 EMAGE:9646 EMAGE:9646 EMAGE:9646 EMAGE:9646
"Pseudo-wholemount" of euxassay_018547. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018547_01 euxassay_018547_02 euxassay_018547_03 euxassay_018547_04
EMAGE:9646 EMAGE:9646 EMAGE:9646 EMAGE:9646 EMAGE:9646
euxassay_018547_05 euxassay_018547_06 euxassay_018547_07 euxassay_018547_08 euxassay_018547_09
EMAGE:9646 EMAGE:9646 EMAGE:9646 EMAGE:9646 EMAGE:9646
euxassay_018547_10 euxassay_018547_11 euxassay_018547_12 euxassay_018547_13 euxassay_018547_14
EMAGE:9646 EMAGE:9646 EMAGE:9646 EMAGE:9646 EMAGE:9646
euxassay_018547_15 euxassay_018547_16 euxassay_018547_17 euxassay_018547_18 euxassay_018547_19
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euxassay_018547_20 euxassay_018547_21 euxassay_018547_22 euxassay_018547_23 euxassay_018547_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9646Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9646_wholemount_strong.wlz
9646_wholemount_moderate.wlz
9646_wholemount_weak.wlz
9646_wholemount_possible.wlz
9646_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9646_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50058
Entity Detected:Aco2, aconitase 2, mitochondrial ( MGI:87880)
Sequence:sense strand is shown

>T50058
AGGACGGCAAGAAGTTCAAGTTGGAGGCTCCAGATGCAGACGAGCTTCCCCGATCGGACTTTGATCCAGG
GCAGGACACATACCAGCACCCCCCTAAGGACAGCAGTGGGCAGCGGGTGGACGTGAGCCCTACCAGCCAG
CGCCTGCAGCTCCTGGAGCCTTTCGACAAGTGGGACGGCAAAGACCTCGAGGACCTGCAAATCCTCATCA
AGGTCAAAGGGAAGTGCACCACAGACCACATTTCTGCTGCCGGCCCCTGGCTCAAGTTCCGTGGGCATCT
GGATAACATCTCTAACAACCTGCTCATCGGTGCCATCAACATCGAAAATGGCAAAGCCAACTCTGTACGC
AATGCTGTCACCCAGGAGTTTGGCCCTGTACCTGACACTGCTCGCTACTACAAGAAACATGGCATCAGGT
GGGTGGTGATTGGAGATGAGAACTATGGTGAGGGCTCAAGCCGGGAGCATGCAGCACTGGAGCCTCGCCA
CCTTGGGGGCCGGGCCATCATCACCAAGAGCTTCGCCAGGATCCACGAAACCAATCTAAAGAAGCAGGGC
TTGCTGCCCCTCACCTTCGCTGACCCCTCCGACTATAACAAGATTCATCCAGTGGACAAGCTGACCATTC
AAGGCCTGAAGGACTTTGCCCCTGGAAAGCCTCTGAAGTGCGTCATCAAGCACCCCAATGGGACCCAGGA
GACCATCCTCCTGAACCACACCTTCAACGAGACCCAGATCGAGTGGTTCCGTGCAGGCAGTGCCCTCAAC
AGGATGAAGGAGCTGCAGCAGTGAGGAGCCAGGCTGGCTGTCCTGACCCACTCTGGACCCTGCTGGATGT
CACTTTTGTCACAGGTCCATGTGCATCATCAGAGCCTAATCTGGTCTGTGCAGCATGACCTCCTGCTCTA
AGATGGTGACCAGACCTGCTT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AGGACGGCAAGAAGTTCAAG; Reverse Primer - name:unspecified, sequence:AAGCAGGTCTGGTCACCATC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9645 same embryo
 EMAGE:9644 same embryo
 EurExpress:euxassay_018547 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS