EMAGE mouse gene expression spatial database

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EMAGE:9672

Zfp295 zinc finger protein 295 ( MGI:1927240)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018487. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018487_01	euxassay_018487_02	euxassay_018487_03	euxassay_018487_04

euxassay_018487_05	euxassay_018487_06	euxassay_018487_07	euxassay_018487_08	euxassay_018487_09

euxassay_018487_10	euxassay_018487_11	euxassay_018487_12	euxassay_018487_13	euxassay_018487_14

euxassay_018487_15	euxassay_018487_16	euxassay_018487_17	euxassay_018487_18	euxassay_018487_19

euxassay_018487_20	euxassay_018487_21	euxassay_018487_22	euxassay_018487_23	euxassay_018487_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9672_wholemount_strong.wlz

9672_wholemount_moderate.wlz

9672_wholemount_weak.wlz

9672_wholemount_possible.wlz

9672_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9672_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T51231
Entity Detected:	Zfp295, zinc finger protein 295 ( MGI:1927240)
Sequence:	sense strand is shown >T51231 GAAGTGTCATTGTGTGTCAGGGCAGAAGTGAAGTGCCAGGGAAAGCCAGTGGTCCAGCCATGCAGGACCT CAGCCATACTGCCCGGGCCTCCCCCAGTGCTTCAGTCAAGACCACCATCAGTAAGCCACATGTGGCCAAG CCGCCAGAGCAGCTTCATAGTCTGCCCTTAACTGAAAAGAGCTGGACAAAGGATAGTGCTGCAGTATATG CAAAGTCTCTGGAACAGGCTGGGGCTTTGGATGATCCTAATAGGGGCAGTTTGGTAAAGAGAAATACAGT CCTGCCCCCAAAGCCTTCACAGGACAGGGAGGCCACAGATGATAAACCAGGGGTGAGCAGCCAGCTTCCC AAGGGGAAAGCTATAGAGCTGGCTCTGAAGAGACCACGGCCCCCTGTTCTGTCTCTTCATAGCTCATCAG AGACTCCATATCTCCTAAAAGAAACTAGCAAAGGAGGCGGTCAGGGAGAGGATAGGAACTTGCTCTACTA CTCTAAGCTAGGGCTGGTGGTCCCTTCTGGTGGGCCTGCTTCTGCAAACCAGAGCATTGACAGAAGTGGC CCACTAGTGAAAAGCCTCCTCAGGCGGTCACTGTCTATGGACAGCCAGGTTCCTGTTTACTCCCCCTCCA TAGATTTGAAGTCATCCCAGGGATCATCCACAGCGGCAAATGAGGCACCGGGTAGTGTGTTCTGTTCGAT GTCTCAAAAGTCATCTTTAAAAGATTGCAGTGAAAAAAAAGCTCTGGATGACAGGCCTCAAGTGCTCCAG CCTCATCGCCTCAGGTCCTTTAGTGCTTCCCAGTCAACAGACAGGGAGGAAGCCTCCCCTGTGACTGAGG TACGCATTAAGACGGAACCCAGCAGCCCACTGTCAGACCCCTCAGACATCATCCGGGTCACTGTGGGAGG AGACGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGCTACGAGAGACCTGCCCCTCAAAACA GAGGAAGACCAGAGAGATATGAGCAGACTCCCAGCAAAGAGAAGGTTCCAGACAGACAGAAGGTCACCCT TGAAGAAGGCAAAGGCAAATGAACACGGGCCTGCTGTCTCAGAAGAGAACTGTGAAGAGGGCAGGAGCCC TCCTTCCCTTGACAGCAACTTCCCAGATTCTGACTTAAACAGAGAGGAGTTTGGTGAGTTGGAGGGGACG AGACCAAACAAAAAATTTAAATGCAAACATTGCCTTAAGATTTTTAGATCAACTGCGGGTCTTCACCGCC ATGTTAACATGTACCATAACCCAGAGAAGCCTTACGCTTGTGACATCTGTCACAAGAGGTTTCACACCAA CTTCAAAGTGTGGACACACTGTCAGACCCAACACGGCATAGTGAAGAACCCATCGCCAGCCTCTAGTTCC CATGCAGTTTTGGATGAGAAATTCCAAAGAAAGCTGATTGACATAGTGAGAGAGAGGGAGATTAAGAAGG CCCTGATCATTAAGCTGAGGCGCAGCAAGCCTGGCTTCCAGGGACAGAGTAGCTCCCCAGCACAGCAAGT CATCAAGAGGAACTTGCGCTCCCGAGCCAAAGGGGCCTACATTTGTGCCTACTGTGGCAAGGCATACCGC TTTCTCTCTCAGTTTAAGCAGCACATAAAGATGCACCCAGGAGAGAGGCCCCTCGGAGTAAGCAGAGCTG CTAAGCCAAAAGAGCGGGCTCTGGCACGCGCGGTAGAGAACAAGGAGGTTTACCCGTGCCGCCTCTGTAA TGCTAAGCTCTCTTCTCTTCTAGAGCAAGGCAACCACGAGCGCTTGTGCCGGAACGCCACCGTTTGCCCT TACTGCAGCCTCAGGTTCTTCTCTCCCGCACTGAAGCAGGAACATGAGGACAGGTGTGAGTACAAAAAGC TGACTTGCCTGGAGTGCATGCGCACCTTCAAGTCCTCCTTCAGCATCTGGCGGCACCAGGTAGAAGTGCA TAACCAGAACAACATGGCTTTAGCAGAGAACTTCGCCCTG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9671 same embryo
	EMAGE:9670 same embryo
	EMAGE:9673 same embryo
	EurExpress:euxassay_018487 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source