EMAGE mouse gene expression spatial database

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EMAGE:97

Hoxb2 homeobox B2 ( MGI:96183)
TS11
in situ hybridisation

Data Images


Fig 1B (left hand embryo). Conlon & Rossant, 1992 [PMID:1363087] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

This embryo is described by the authors as "headfold" stage.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

97_wholemount_strong_3D_1.wlz

97_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:97_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

detected

graded

The expression of Hox-2.8 gradually decreased anteriorly, ending slightly anterior to the node

node

detected

graded

embryo mesoderm

detected

graded

embryo ectoderm

detected

graded

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1289712
Entity Detected:	Hoxb2, homeobox B2 ( MGI:96183)
Notes:	The Hoxb2 (Hox-2.8) probe used in this study by Conlon & Rossant, 1992 [PMID:1363087] is described as "plasmid 865, a 3 kb Hox-2.8 fragment in pBluescript KS" and was a gift from R.Krumlauf.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD1
Theiler Stage:	TS11
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Conlon & Rossant, 1992 [PMID:1363087] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:1363087] Conlon RA, Rossant J 1992 Exogenous retinoic acid rapidly induces anterior ectopic expression of murine Hox-2 genes in vivo. Development (116):357-68
Links:	MGI:1289738 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source