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EMAGE:9713

Idh3b isocitrate dehydrogenase 3 (NAD+) beta ( MGI:2158650)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9713 EMAGE:9713 EMAGE:9713 EMAGE:9713 EMAGE:9713
"Pseudo-wholemount" of euxassay_018530. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018530_01 euxassay_018530_02 euxassay_018530_03 euxassay_018530_04
EMAGE:9713 EMAGE:9713 EMAGE:9713 EMAGE:9713 EMAGE:9713
euxassay_018530_05 euxassay_018530_06 euxassay_018530_07 euxassay_018530_08 euxassay_018530_09
EMAGE:9713 EMAGE:9713 EMAGE:9713 EMAGE:9713 EMAGE:9713
euxassay_018530_10 euxassay_018530_11 euxassay_018530_12 euxassay_018530_13 euxassay_018530_14
EMAGE:9713 EMAGE:9713 EMAGE:9713 EMAGE:9713 EMAGE:9713
euxassay_018530_15 euxassay_018530_16 euxassay_018530_17 euxassay_018530_18 euxassay_018530_19
EMAGE:9713 EMAGE:9713 EMAGE:9713 EMAGE:9713 EMAGE:9713
euxassay_018530_20 euxassay_018530_21 euxassay_018530_22 euxassay_018530_23 euxassay_018530_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9713Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9713_wholemount_strong.wlz
9713_wholemount_moderate.wlz
9713_wholemount_weak.wlz
9713_wholemount_possible.wlz
9713_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9713_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50566
Entity Detected:Idh3b, isocitrate dehydrogenase 3 (NAD+) beta ( MGI:2158650)
Sequence:sense strand is shown

>T50566
ATTTGTTTGCCAACGTAGTCCACGTGAAGTCACTTCCTGGATACAAGACTCGGCACAACAATCTAGACCT
GGTTATCATTCGAGAGCAGACAGAAGGGGAGTATAGCTCTCTGGAACATGAGAGCGCCAAGGGTGTCATT
GAGTGCCTGAAGATCGTCACTCGCACCAAGTCTCAGAGGATTGCAAAGTTTGCGTTCGACTATGCCACCA
AGAAAGGGCGGAGCAAGGTCACAGCCGTCCATAAAGCCAACATCATGAAACTAGGGGATGGCTTGTTCTT
GCAGTGCTGTGAGGAAGTTGCTGAACTGTACCCTAAAATCAAGTTTGAAACCATGATCATAGACAACTGC
TGCATGCAGCTGGTGCAGAACCCTTACCAGTTTGATGTGCTCGTGATGCCCAATCTCTATGGCAACATAA
TTGACAATCTGGCTGCTGGCCTTGTTGGGGGAGCTGGCGTGGTTCCTGGGGAGAGCTACAGTGCAGAGTA
TGCAGTTTTTGAGACGGGTGCTCGGCACCCATTTGCCCAGGCAGTGGGCAGGAATATAGCCAACCCCACA
GCCATGCTGCTGTCCGCCACCAACATGCTGCGGCATCTCAATCTTGAGTATCACTCCAGCATGATTGCAG
ATGCAGTGAAGAAAGTGATCAAAGCTGGCAAGGTACGGACTCGAGACATGGGAGGCTACAGCACCACAAC
TGACTTCATCAAGTCTGTCATCGGCCACCTGCACCCCCATGGGGGCTAGAGCCCTTACTCCCTCCAATTT
CAAAAGGACCATGCTTCGTATACATCCCTTCAGTACAATGGACCAGAAGAGAACATCTAGACAGTAGACT
ATAATAGCTTTTCTGAGGCTAGGCTGTCCTGGGGGCTGGTGTTAAGGGTATCTCAAAGGGTGGGTTGTTG
CGACAAGGCCCAGACCCTAAGATGATAACTTTTTCCCACAGGTTCGAACCTCAGATATGGGTGGTTAT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ATTTGTTTGCCAACGTAGTC; Reverse Primer - name:unspecified, sequence:ATAACCACCCATATCTGAGG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9714 same embryo
 EurExpress:euxassay_018530 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS