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EMAGE:9717

Atp9a ATPase, class II, type 9A ( MGI:1330826)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9717 EMAGE:9717 EMAGE:9717 EMAGE:9717 EMAGE:9717
"Pseudo-wholemount" of euxassay_018413. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018413_01 euxassay_018413_02 euxassay_018413_03 euxassay_018413_04
EMAGE:9717 EMAGE:9717 EMAGE:9717 EMAGE:9717 EMAGE:9717
euxassay_018413_05 euxassay_018413_06 euxassay_018413_07 euxassay_018413_08 euxassay_018413_09
EMAGE:9717 EMAGE:9717 EMAGE:9717 EMAGE:9717 EMAGE:9717
euxassay_018413_10 euxassay_018413_11 euxassay_018413_12 euxassay_018413_13 euxassay_018413_14
EMAGE:9717 EMAGE:9717 EMAGE:9717 EMAGE:9717 EMAGE:9717
euxassay_018413_15 euxassay_018413_16 euxassay_018413_17 euxassay_018413_18 euxassay_018413_19
EMAGE:9717 EMAGE:9717 EMAGE:9717 EMAGE:9717 EMAGE:9717
euxassay_018413_20 euxassay_018413_21 euxassay_018413_22 euxassay_018413_23 euxassay_018413_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9717Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9717_wholemount_strong.wlz
9717_wholemount_moderate.wlz
9717_wholemount_weak.wlz
9717_wholemount_possible.wlz
9717_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9717_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50133
Entity Detected:Atp9a, ATPase, class II, type 9A ( MGI:1330826)
Sequence:sense strand is shown

>T50133
ACATGACGGACAGCATCCCGCTGCAGCCCGTGCGCCACAAGAAGCGGGTGGACAGTAGGCCGCGCGCGGG
GTGCTGTGAGTGGCTGAGATGTTGCGGTGGAGGGGAGCCCAGGCCCCGTACTGTCTGGTTGGGACACCCC
GAGAAGAGGGACCAGCGGTACCCTCGAAATGTCATCAACAACCAGAAGTACAATTTCTTCACATTTCTTC
CTGGGGTGTTGTTCAGCCAGTTCAGATACTTCTTCAACTTCTACTTCCTGCTTCTCGCCTGCTCGCAGTT
CGTCCCAGAGATGAGGCTTGGCGCCCTGTACACCTACTGGGTTCCTCTGGGCTTCGTGCTGGCTGTCACC
ATCATCCGTGAGGCAGTAGAGGAGATCCGATGTTATGTGCGTGACAAGGAGATGAACTCCCAGGTCTACA
GCCGGCTCACGTCACGAGGGACCGTGAAGGTGAAGAGTTCAAACATCCAGGTGGGAGACCTCATCCTTGT
GGAAAAGAACCAGCGGGTCCCTGCTGACATGATCTTCCTGAGGACGTCAGAGAAAAACGGCTCTTGCTTC
TTGCGCACGGATCAGCTGGATGGAGAGACAGACTGGAAGCTTCGGCTCCCGGTGGCCTGCACACAGAGGC
TTCCCACGGCTGCTGACCTCCTGCAGATTCGGTCCTATGTGTACGCTGAAGAGCCCAACATCGACATTCA
CAACTTCCTGGGGACTTTCACCAGGGAAGACAGTGACCCTCCGATCAGTGAGAGTCTGAGCATTGAGAAC
ACGCTGTGGGCCGGCACCGTCATAGCATCAGGCACTGTTGTAGGCGTTGTTCTCTACACTGGCAGAAA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ACATGACGGACAGCATCCCG; Reverse Primer - name:unspecified, sequence:TTTCTGCCAGTGTAGAGAAC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9716 same embryo
 EMAGE:9715 same embryo
 EMAGE:9718 same embryo
 EurExpress:euxassay_018413 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS