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EMAGE:9734

Pgam1 phosphoglycerate mutase 1 ( MGI:97552)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9734 EMAGE:9734 EMAGE:9734 EMAGE:9734 EMAGE:9734
"Pseudo-wholemount" of euxassay_018426. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018426_01 euxassay_018426_02 euxassay_018426_03 euxassay_018426_04
EMAGE:9734 EMAGE:9734 EMAGE:9734 EMAGE:9734 EMAGE:9734
euxassay_018426_05 euxassay_018426_06 euxassay_018426_07 euxassay_018426_08 euxassay_018426_09
EMAGE:9734 EMAGE:9734 EMAGE:9734 EMAGE:9734 EMAGE:9734
euxassay_018426_10 euxassay_018426_11 euxassay_018426_12 euxassay_018426_13 euxassay_018426_14
EMAGE:9734 EMAGE:9734 EMAGE:9734 EMAGE:9734 EMAGE:9734
euxassay_018426_15 euxassay_018426_16 euxassay_018426_17 euxassay_018426_18 euxassay_018426_19
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euxassay_018426_20 euxassay_018426_21 euxassay_018426_22 euxassay_018426_23 euxassay_018426_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9734Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9734_wholemount_strong.wlz
9734_wholemount_moderate.wlz
9734_wholemount_weak.wlz
9734_wholemount_possible.wlz
9734_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9734_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50828
Entity Detected:Pgam1, phosphoglycerate mutase 1 ( MGI:97552)
Sequence:sense strand is shown

>T50828
AAGGTGAAGAGGCAGACTCCTACCCCGACACCCTCCCTGCCTGTTCCTGCCTCCTGCACCCTCCCCAGCA
CCTGCCACACTGACTATGTCTTTAGGATTTTAAGTTTAAGTTGTAGCTGCAGATGGGGGCCTGTGGTCCC
GTTTTCATTGTAGTATTTTATCCCCTGCACCCACTCCCTTCATAGAATCTAGTCAGAATAACGCCTCTGG
GGGTGCAGGTTCTCGGACCACATCGAGGGACAGCTCCCTGTACCAGGAGAGTTGAGAGATAGTAACTATA
GTTTTTCTTAGGTCTTTGTTACTAAGGGTCTGCTTAGGAAGAATTCAGGGAGGAACTGTGCTGTGGTGTG
ACCAATGAAAAGCTGCAGTCCAGTTTTGTCCTGGAACCTGTCCTGCACTCTTCCTGCAGTTAGGTAACTG
GGGGCATAGTCATTCCAGTGGAGAGTGGAAGTAACCTCATGGTGATTTTTAACCCCTAATTCTCTGACCC
TAAAGGAGCTGCTGGCCCTAGAAGGTTGGGATCAATCTAGTCAAGTCTATGTTACACTTACTTTTACAAA
AAAAAGTATATATACATATTATCAGCACACAAAAGCAACTCTGAGGTTGCTAGTGACATTTGAAACAGTC
CTGTGTGGTCACCAGAAAATAAGCCATTCCTCATATCAGTGGGATAAACTCCTTGTACCTCTTGCTGTGT
TTGGATCCCTTGGCCTTGTTTGTAGAGGTGAAGTACCCCTGGATCATGTCCGAGTTTTTGTCTTTCACTG
TCTGAATCATGTTCCAGCTGCTTGACCCTACCGTGTGGGTTCAGAGCTCGTTTGGGCGTAACAACT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AAGGTGAAGAGGCAGACTCC; Reverse Primer - name:unspecified, sequence:AGTTGTTACGCCCAAACGAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9735 same embryo
 EurExpress:euxassay_018426 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS