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EMAGE:9745

Grp gastrin releasing peptide ( MGI:95833)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9745 EMAGE:9745 EMAGE:9745 EMAGE:9745 EMAGE:9745
"Pseudo-wholemount" of euxassay_018407. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018407_01 euxassay_018407_02 euxassay_018407_03 euxassay_018407_04
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euxassay_018407_10 euxassay_018407_11 euxassay_018407_12 euxassay_018407_13 euxassay_018407_14
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euxassay_018407_20 euxassay_018407_21 euxassay_018407_22 euxassay_018407_23 euxassay_018407_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9745Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9745_wholemount_strong.wlz
9745_wholemount_moderate.wlz
9745_wholemount_weak.wlz
9745_wholemount_possible.wlz
9745_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9745_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50439
Entity Detected:Grp, gastrin releasing peptide ( MGI:95833)
Sequence:sense strand is shown

>T50439
TAGTCGAGAGCTCTGAGGGTTTCCCTCCAGGACCATGCGCGGCTCGGAGCTCTCGCTCTTGCTGTTGGCT
CTGGTCCTCTGCCAGGCGCCCCGGGGGCCAGCTGCCCCGGTGTCGACAGGCGCAGGTGGAGGCACGGTCC
TGGCTAAGATGTATCCGCGCGGCAGTCACTGGGCTGTGGGACACTTAATGGGAAAAAAGAGCACGGATGA
ATCCCCGTCCCTGTATGCGGCTGACAGAGACGGCCTGAAGGAGCAGCTGAGGGGGTACGTCCGCTGGGAA
GAAGCTGCAAGGGATTTGCTGGACCTCCTAGAAGCTGCTGGGAACCAAAGCCACCAGCCACCTCAGCATC
CGCCTCTCAGTCTCCAGCCTACTTGGGATCCCGAGGACGGCAGCTACTTTAACGACGTTCAAACCGCTAA
GTTGGTAGACTCTCTGCTCCAGGTTCTCAAGGAAAAGGGAGGAACTGCCAGCTGAAGGGACAACGACAAG
GGCGGCTTCCAAGGAGCAAAACAAAACCCTTCAAAGACTGCCTTCTGCAAACGTCAACTCTACTGGACCA
TCAACAAGATTTCCTTTGTGCAATTTACTTAACTATTCTCCTTATCTTTCAACCGCAACTAAACTCGTGA
TTTTTCGAGCAGCATCTTCTGGTTTGAACTTGTTTCTGTGAACCACTCACTACTTAGAAAGAGCTTTCCA
ATGAACGCTTTTTCCAGCTAGGCTTTTTGTTGATTAGATTCAGGGCTCCAATTTGCTACCATTCACA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TAGTCGAGAGCTCTGAGGGTTT; Reverse Primer - name:unspecified, sequence:TGTGAATGGTAGCAAATTGGAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9744 same embryo
 EMAGE:9743 same embryo
 EurExpress:euxassay_018407 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS