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EMAGE:9801

Hecw1 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 ( MGI:2444115)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9801 EMAGE:9801 EMAGE:9801 EMAGE:9801 EMAGE:9801
"Pseudo-wholemount" of euxassay_018652. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018652_01 euxassay_018652_02 euxassay_018652_03 euxassay_018652_04
EMAGE:9801 EMAGE:9801 EMAGE:9801 EMAGE:9801 EMAGE:9801
euxassay_018652_05 euxassay_018652_06 euxassay_018652_07 euxassay_018652_08 euxassay_018652_09
EMAGE:9801 EMAGE:9801 EMAGE:9801 EMAGE:9801 EMAGE:9801
euxassay_018652_10 euxassay_018652_11 euxassay_018652_12 euxassay_018652_13 euxassay_018652_14
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euxassay_018652_15 euxassay_018652_16 euxassay_018652_17 euxassay_018652_18 euxassay_018652_19
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euxassay_018652_20 euxassay_018652_21 euxassay_018652_22 euxassay_018652_23 euxassay_018652_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9801Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9801_wholemount_strong.wlz
9801_wholemount_moderate.wlz
9801_wholemount_weak.wlz
9801_wholemount_possible.wlz
9801_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9801_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50522
Entity Detected:Hecw1, HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 ( MGI:2444115)
Sequence:sense strand is shown

>T50522
CTAAGATCTGCTTCAAATACTACCACGGAGTAAGTGGGGCCCTGCGAGCTACAACCCCCAGTGTCACTGT
CAAGAACTCAGCAGCCCCTATTTTTAAAGGTATTGGCTCTGAAGAGACTGCACAAAGTCAAGGAAGCCGG
AGGCTGATCAGCTTCTCCCTCTCAGATTTCCAGGCCATGGGATTGAAGAAAGGGATGTTTTTCAACCCCG
ACCCTTATCTGAAGATTTCCATTCAACCTGGGAAACATAGCATCTTCCCAGCCCTCCCTCATCATGGACA
GGAAAGGAGATCCACGATCATAGGCAACACTGTAAACCCCATCTGGCAGGCAGAGCATTTCAGTTTCGTG
TCTCTGCCAACCGATGTGCTGGAAATTGAAGTGAAGGACAAGTTTGCCAAGAGTCGTCCCATCATCAAGC
GTTTCTTGGGAAAGCTGTCAATGCCTGTTCAGAGGCTGCTGGAGAGACACGCCATAGGGTAAACTGTGAC
TGGGTGGCTGTGCTTCCACTTACAGGCCATGCCCAGGAAGGCTTGTAAGCTGCCATTACAAAGTCTGATG
GAAAGACACCTAAAATGGTTTCTAGTTTCGGGGCTTTCCCCACCAATATCATTTCCTGGACCATGGTATT
CAGTTAAATCCTTCCCCTTCTTATACTTGTGTAGTTTGTATAATACATATTAAAGAGTATAGAGAAATCA
TAGACAGTATTGAAAAATAATACACATTTTCCTGTGTAAGTATAGATTTAAACTTTGCCATTGCTGTGGA
AAATAACTCATTCCTTCACCTGCCTTGTATGTAGGATTTAACCACATACTGCACTTCCATACGAAGATGG
AATCATGTTTCTTGGAGAACATTTCTCA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AGCTGTCAATGCCTGTTCAG; Reverse Primer - name:unspecified, sequence:TCCTCCTCCAAGCCATCTTC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9800 same embryo
 EMAGE:9802 same embryo
 EMAGE:9803 same embryo
 EurExpress:euxassay_018652 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS