Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:9818

Akt1 thymoma viral proto-oncogene 1 ( MGI:87986)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9818 EMAGE:9818 EMAGE:9818 EMAGE:9818 EMAGE:9818
"Pseudo-wholemount" of euxassay_018752. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018752_01 euxassay_018752_02 euxassay_018752_03 euxassay_018752_04
EMAGE:9818 EMAGE:9818 EMAGE:9818 EMAGE:9818 EMAGE:9818
euxassay_018752_05 euxassay_018752_06 euxassay_018752_07 euxassay_018752_08 euxassay_018752_09
EMAGE:9818 EMAGE:9818 EMAGE:9818 EMAGE:9818 EMAGE:9818
euxassay_018752_10 euxassay_018752_11 euxassay_018752_12 euxassay_018752_13 euxassay_018752_14
EMAGE:9818 EMAGE:9818 EMAGE:9818 EMAGE:9818 EMAGE:9818
euxassay_018752_15 euxassay_018752_16 euxassay_018752_17 euxassay_018752_18 euxassay_018752_19
EMAGE:9818 EMAGE:9818 EMAGE:9818 EMAGE:9818 EMAGE:9818
euxassay_018752_20 euxassay_018752_21 euxassay_018752_22 euxassay_018752_23 euxassay_018752_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9818Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9818_wholemount_strong.wlz
9818_wholemount_moderate.wlz
9818_wholemount_weak.wlz
9818_wholemount_possible.wlz
9818_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9818_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T51121
Entity Detected:Akt1, thymoma viral proto-oncogene 1 ( MGI:87986)
Sequence:sense strand is shown

>T51121
TCTAGGCATCCCTTCCTTACGGCCCTCAAGTACTCATTCCAGACCCACGACCGCCTCTGCTTTGTCATGG
AGTATGCCAACGGGGGCGAGCTCTTCTTCCACCTGTCTCGAGAGCGCGTGTTCTCCGAGGACCGGGCCCG
CTTCTATGGTGCGGAGATTGTGTCTGCCCTGGACTACTTGCACTCCGAGAAGAACGTGGTGTACCGGGAC
CTGAAGCTGGAGAACCTCATGCTGGACAAGGACGGGCACATCAAGATAACGGACTTCGGGCTGTGCAAGG
AGGGGATCAAGGATGGTGCCACTATGAAGACATTCTGCGGAACGCCGGAGTACCTGGCCCCTGAGGTGCT
GGAGGACAACGACTACGGCCGTGCAGTGGACTGGTGGGGGCTGGGCGTGGTCATGTATGAGATGATGTGT
GGCCGCCTGCCCTTCTACAACCAGGACCACGAGAAGCTGTTCGAGCTGATCCTCATGGAGGAGATCCGCT
TCCCGCGCACACTCGGCCCTGAGGCCAAGTCCCTGCTCTCCGGGCTGCTCAAGAAGGACCCTACACAGAG
GCTCGGTGGGGGCTCTGAGGATGCCAAGGAGATCATGCAGCACCGGTTCTTTGCCAACATCGTGTGGCAG
GATGTGTATGAGAAGAAGCTGAGCCCACCTTTCAAGCCCCAGGTCACCTCTGAGACTGACACCAGGTATT
TCGATGAGGAGTTCACAGCTCAGATGATCACCATCACGCCGCCTGATCAAGATGACAGCATGGAGTGTGT
GGACAGTGAGCGGAGGCCGCACTTCCCCCAGTTCTCCTACTCAGCCAGTGGCACAGCCTGAGGCCTGGGG
CAGCGGCTGGCAGCTCCACGCTCCTCTGCATTGCCGAGTCCAGAAGCCCCGCATGGATCATCTGAACCTG
ATGTTTTGTTTCTCGGATGCGCTGGGGAGGAACCTTGCCAGCCTCCAGGACCAGGGGAGGATGTTTCTAC
TGTGGGCAGCAGCCTACCTCCCAGCCAGGTCAGGAGGAAAACTATCCTGGGGTTTTTCTTAATTTATTTC
ATCCAGTTTGAGACCACACATGTGGCCTCAGTGCCCAGAACAATT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TCTAGGCATCCCTTCCTTACGG; Reverse Primer - name:unspecified, sequence:AATTGTTCTGGGCACTGAGGC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9816 same embryo
 EMAGE:9817 same embryo
 EurExpress:euxassay_018752 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS