EMAGE mouse gene expression spatial database

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EMAGE:9874

Ncbp2 nuclear cap binding protein subunit 2 ( MGI:1915342)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008123_01	euxassay_008123_02	euxassay_008123_03	euxassay_008123_04	euxassay_008123_05

euxassay_008123_06	euxassay_008123_07	euxassay_008123_08	euxassay_008123_09	euxassay_008123_10

euxassay_008123_11	euxassay_008123_12	euxassay_008123_13	euxassay_008123_14	euxassay_008123_15

euxassay_008123_16	euxassay_008123_17	euxassay_008123_18	euxassay_008123_19	euxassay_008123_20

euxassay_008123_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9874_wholemount_strong.wlz

9874_wholemount_moderate.wlz

9874_wholemount_weak.wlz

9874_wholemount_possible.wlz

9874_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9874_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30
Entity Detected:	Ncbp2, nuclear cap binding protein subunit 2 ( MGI:1915342)
Sequence:	sense strand is shown >T30 TTTTTTTTTTTTTTCTTTGGATTCTGTCTTTTAATATTTCAGAGGAAAACCAGAGGTGGCTTCCAGTACA GCAGAGACACTGATGAGACCTTGGGTAACGTTCTCCAGCATCACCACTGTGTTGATGTACCGTGACCTGT GTACTTGGGGCTCACCCCACTCACTGTTTTTGTGCCAGTTTCCCATAGCCGCCTCTTCCAGCATCGTAGT CCTCCCGATACTCGTCCCGAACCTGACCCCCAGACCGTCCACGTCCATACTGCCTGCCCTCCTTAAAGCC TGCATCCCAGTCTGTCCGTATGATCCGGTCATCCANACGAGTTCCGTTTATGTACCGCATTGCGTTCTCC GCATCTGCTCTTGAATAGTATTCCACAAAACAGAACCCACATGCTGTTTTCTTCATTTTATCCAGACCCA TGATGATCTTCTTTATATCACCACTTTTGCTGAAGAGTTCATAAATCTGTTCTTCAGTTGTATAAAAGGA AAGATTTCCAACATACAACGTAC
Notes:	The probe template was PCR amplified from IMAGE:944189 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:944189 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9875 same embryo
	EurExpress:euxassay_008123 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source