EMAGE mouse gene expression spatial database

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EMAGE:9909

Rimkla ribosomal modification protein rimK-like family member A ( MGI:3040686)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008155. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008155_01	euxassay_008155_02	euxassay_008155_03	euxassay_008155_04

euxassay_008155_05	euxassay_008155_06	euxassay_008155_07	euxassay_008155_08	euxassay_008155_09

euxassay_008155_10	euxassay_008155_11	euxassay_008155_12	euxassay_008155_13	euxassay_008155_14

euxassay_008155_15	euxassay_008155_16	euxassay_008155_17	euxassay_008155_18	euxassay_008155_19

euxassay_008155_20	euxassay_008155_21	euxassay_008155_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9909_wholemount_strong.wlz

9909_wholemount_moderate.wlz

9909_wholemount_weak.wlz

9909_wholemount_possible.wlz

9909_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9909_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 15 16 17 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 05 15

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 16

ventral grey horn

moderate

regional

moderate expression: see section 08 12 weak expression: see section 09

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 13 14 weak expression: see section 04 05 06 07

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T64
Entity Detected:	Rimkla, ribosomal modification protein rimK-like family member A ( MGI:3040686)
Sequence:	sense strand is shown >T64 CTTTCTAGGTGGTGTGGGTGTCAAGTGCCCACTGACCGAGCAAGGCAAGCAGCTGGCTATCCAGGTGTCC AACATCCTGGGCATGGATTTCTGTGGCATCGACCTTCTTATCATGGATGACGGCTCCTTCACGGTGTGTG AGGCCAACGCCAATGTCGGCTTCCTGGCCTTCGACCAGGCATGCAACTTAGATGTGGGGGCAATCATTGC AGACTATGCTATGTCCCTGCTGCCAAACAGGCAGACTGGGAAGATGGCCATCCTCCCGGGACTGGCAAGT CCCAGGGAGAAGAACGAGCCAAATGGTTGCGTTTCAGCTCAGGGAGTTGCAGAGAGTGTCTATGCCATCA CCAATGGGTCTACCTCTAGTGAGAGTGAGCCTGAACTGGGAGAGGCCCGGGATTCCTTAGTAAAAACAAT GGGGGCCCCGCCTGCGCATGTTGCCCAAGCCTGGTTACAGCATTAACAGGATTGCGTCACGATTCCAGTG AATTCCTGCTTTCTTGGCAGCA
Notes:	The probe template was PCR amplified from IMAGE:1123503 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1123503 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9908 same embryo
	EMAGE:9907 same embryo
	EurExpress:euxassay_008155 same experiment
	MGI:4827744 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source