EMAGE mouse gene expression spatial database

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EMAGE:9926

Klhl8 kelch-like 8 (Drosophila) ( MGI:2179430)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008140. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008140_01	euxassay_008140_02	euxassay_008140_03	euxassay_008140_04

euxassay_008140_05	euxassay_008140_06	euxassay_008140_07	euxassay_008140_08	euxassay_008140_09

euxassay_008140_10	euxassay_008140_11	euxassay_008140_12	euxassay_008140_13	euxassay_008140_14

euxassay_008140_15	euxassay_008140_16	euxassay_008140_17	euxassay_008140_18	euxassay_008140_19

euxassay_008140_20	euxassay_008140_21	euxassay_008140_22	euxassay_008140_23	euxassay_008140_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9926_wholemount_strong.wlz

9926_wholemount_moderate.wlz

9926_wholemount_weak.wlz

9926_wholemount_possible.wlz

9926_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9926_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14

telencephalon marginal layer

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23 24

olfactory cortex marginal layer

weak

regional

weak expression: see section 07 08 09 10 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T69
Entity Detected:	Klhl8, kelch-like 8 (Drosophila) ( MGI:2179430)
Sequence:	sense strand is shown >T69 ACCTAAACACCGTGGAGGCGTTTGACCCCGTGCTGAACAAGTGGGAGCTGGTCGGACCTGTGTCTCACTG CCGAGCTGGTGCCGGTGTGGCCGTGTGTGACTGTTTAACTAGCCAGATCCGAGACGTGGGTCACGGGTCC ACTAATGTGGTTGACTGTATGTGATGAAGTCCCAGCAACAAGGACACAATCCCGTCTGATGGACAGGAAG ACAGCAGGCCTTTTGACTGTGACTGCAGCGACTGTTTTTGAATGTCAGCTGTTGTGAATACACGTCTGTG GACTGACGGGTGAGAGCTGTTGTCATAAAGCCAGGTGAGCTCTTTCCTGCTTCAGCAAACAGTGGAGCGG GCTGGAAGCAGCCCATCTTGAACCCATTCTTGCTCTGAGAGATGTTTGATGTAGTGCCAAACCTTAAAAT AGCTCTTTATTTTGATGAATAAAATGTAATTTATTGAGTTTCATCTATTCTTTTTCCTGAAGAAACTAAC TTCAGGCAGTAGGTTTTTCCCAGAGGAGAGAGATGTCCACTCAT
Notes:	The probe template was PCR amplified from IMAGE:1125156 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1125156 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9925 same embryo
	EurExpress:euxassay_008140 same experiment
	MGI:4825808 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source