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EMAGE:9935

Tada3 transcriptional adaptor 3 ( MGI:1915724)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9935 EMAGE:9935 EMAGE:9935 EMAGE:9935 EMAGE:9935
"Pseudo-wholemount" of euxassay_008186. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008186_01 euxassay_008186_02 euxassay_008186_03 euxassay_008186_04
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euxassay_008186_05 euxassay_008186_06 euxassay_008186_07 euxassay_008186_08 euxassay_008186_09
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euxassay_008186_10 euxassay_008186_11 euxassay_008186_12 euxassay_008186_13 euxassay_008186_14
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euxassay_008186_15 euxassay_008186_16 euxassay_008186_17 euxassay_008186_18 euxassay_008186_19
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euxassay_008186_20 euxassay_008186_21 euxassay_008186_22 euxassay_008186_23 euxassay_008186_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9935Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9935_wholemount_strong.wlz
9935_wholemount_moderate.wlz
9935_wholemount_weak.wlz
9935_wholemount_possible.wlz
9935_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9935_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T219
Entity Detected:Tada3, transcriptional adaptor 3 ( MGI:1915724)
Sequence:sense strand is shown

>T219
GAATATGAATTTACTGATGACCCAATCGACGTGCCTCGGATCCCCAAGAATGATGCTCCCAACAGGTTCT
GGGCCTCTGTGGAGCCCTATTGTGCCGACATCACCAGTGAGGAGGTACGGACACTAGAGGAGCTCCTGAA
GCCCCCAGAAGATGAAGCTGAACATTATAAGATTCCCCCCCTAGGGAAACACTACTCCCAGCGCTGGGCC
CAGGAGGATCTGCTGGAGGAGCAGAAGGACGGCGCTCGGGCAGCAGCTGTGGCTGACAAGAAGAAAGGCC
TCATAGGACCACTGACAGAACTGGACACAAAAGATGTGGATGCCCTGCTGAAGAAGTCTGAGGCCCAGCA
TGAACAGCCAGAAGACGGGTGTCCCTTTGGAGCCCTGACACAGCGCCTGCTGCAGGCCCTAGTGGAGGAA
AATATTATCTCCCCTATGGAGGACTCCCCTATTCCTGACATGTCTGGGAAAGAGTCAGGGGCAGATGGAG
CG
Notes:The probe template was PCR amplified from IMAGE:2649092 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2649092 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9936 same embryo
 EMAGE:9932 same embryo
 EMAGE:9934 same embryo
 EMAGE:9933 same embryo
 EurExpress:euxassay_008186 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS