EMAGE mouse gene expression spatial database

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EMAGE:9941

Dhrs7 dehydrogenase/reductase (SDR family) member 7 ( MGI:1913625)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000191. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000191_01	euxassay_000191_02	euxassay_000191_03	euxassay_000191_04

euxassay_000191_05	euxassay_000191_06	euxassay_000191_07	euxassay_000191_08	euxassay_000191_09

euxassay_000191_10	euxassay_000191_11	euxassay_000191_12	euxassay_000191_13	euxassay_000191_14

euxassay_000191_15	euxassay_000191_16	euxassay_000191_17	euxassay_000191_18	euxassay_000191_19

euxassay_000191_20	euxassay_000191_21	euxassay_000191_22	euxassay_000191_23	euxassay_000191_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9941_wholemount_strong.wlz

9941_wholemount_moderate.wlz

9941_wholemount_weak.wlz

9941_wholemount_possible.wlz

9941_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9941_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

strong

regional

strong expression: see section 16

hypothalamus marginal layer

strong

regional

strong expression: see section 16

hypothalamus ventricular layer

strong

regional

strong expression: see section 15 16

left atrium

strong

homogeneous

strong expression: see section 15

dorsal mesocardium

strong

regional

strong expression: see section 14 15 16

lower jaw alveolar sulcus

strong

homogeneous

strong expression: see section 14 15 16 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T260
Entity Detected:	Dhrs7, dehydrogenase/reductase (SDR family) member 7 ( MGI:1913625)
Sequence:	sense strand is shown >T260 GCAAGGGACAGCGATGAGCTGGGAGCTGCTGTTGTGGCTGCTGGCGCTGTGCGCGCTGATCCTGCCCCTT GTGCAGCTCTTGCGCTTTTTGCGGGCGGATGCGGACCTGACCTTGTTGTGGGCTGAATGGCAAGGGCGAC GCCCAGAATGGGAGCTGACTGACATGGTGGTGTGGGTTACTGGAGCGTCAAGTGGCATTGGCGAGGAGCT AGCTTTCCAGTTATCTAAACTCGGGGTCTCTCTGGTGCTGTCAGCCCGAAGGGCGCAGGAGCTGGAGCGG GTGAAGAGAAGATGCCTGGAAAATGGCAATTTAAAAGAAAAAGATATACTGGTTCTGCCCCTTGACTTGA CCGATACAAGTTCCCATGAGGCAGCCACCAAAGCCGTTCTGCAGGAGTTTGGTAAAATTGACATTTTGGT CAACAACGGTGGAAGAAGCCAGCGTTCCTTAGTTCTAGAAACCAACTTGGATGTCTTCAAGGAGCTGATA AATCTGAACTACATAGGG
Notes:	The probe template was PCR amplified from IMAGE:2654275 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2654275 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9942 same embryo
	EMAGE:9943 same embryo
	EurExpress:euxassay_000191 same experiment
	MGI:4824301 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source