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EMAGE:9949

Slc38a2 solute carrier family 38, member 2 ( MGI:1915010)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9949 EMAGE:9949 EMAGE:9949 EMAGE:9949 EMAGE:9949
"Pseudo-wholemount" of euxassay_019685. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019685_01 euxassay_019685_02 euxassay_019685_03 euxassay_019685_04
EMAGE:9949 EMAGE:9949 EMAGE:9949 EMAGE:9949 EMAGE:9949
euxassay_019685_05 euxassay_019685_06 euxassay_019685_07 euxassay_019685_08 euxassay_019685_09
EMAGE:9949 EMAGE:9949 EMAGE:9949 EMAGE:9949 EMAGE:9949
euxassay_019685_10 euxassay_019685_11 euxassay_019685_12 euxassay_019685_13 euxassay_019685_14
EMAGE:9949 EMAGE:9949 EMAGE:9949 EMAGE:9949 EMAGE:9949
euxassay_019685_15 euxassay_019685_16 euxassay_019685_17 euxassay_019685_18 euxassay_019685_19
EMAGE:9949 EMAGE:9949 EMAGE:9949 EMAGE:9949 EMAGE:9949
euxassay_019685_20 euxassay_019685_21 euxassay_019685_22 euxassay_019685_23 euxassay_019685_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9949Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9949_wholemount_strong.wlz
9949_wholemount_moderate.wlz
9949_wholemount_weak.wlz
9949_wholemount_possible.wlz
9949_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9949_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forelimb digit 1 phalanx
moderate moderate
regionalmoderate expression: see section 02
forelimb digit 2 phalanx
moderate moderate
regionalmoderate expression: see section 02
hindlimb digit 1 phalanx
moderate moderate
regionalmoderate expression: see section 04 05 20 21 weak expression: see section 22
hindlimb digit 2 phalanx
moderate moderate
regionalmoderate expression: see section 05 06 07 19 20 21 weak expression: see section 22
hindlimb digit 3 phalanx
moderate moderate
regionalmoderate expression: see section 07 08 19 20
hindlimb digit 4 phalanx
moderate moderate
regionalmoderate expression: see section 19
hindlimb digit 5 phalanx
moderate moderate
regionalmoderate expression: see section 19 21
adrenal gland
moderate moderate
regionalmoderate expression: see section 09 10 11 17 18 19
submandibular gland primordium
strong strong
regionalstrong expression: see section 06 07 08 09 18 19 20 21
vibrissa
weak weak
regionalweak expression: see section 06 07 08 21 22 23 24
telencephalon meninges
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 13 14 15 16 19 20 21 22 23 24
hindbrain meninges
moderate moderate
regionalmoderate expression: see section 02 03 04 11 12 13 14 15 16
neural retina
weak weak
regionalweak expression: see section 01 02 03 04 23 24
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 11 weak expression: see section 12 16 17 18
lower jaw molar
moderate moderate
regionalmoderate expression: see section 06 07 21
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 11 weak expression: see section 12 13 17 18
upper jaw molar
moderate moderate
regionalmoderate expression: see section 06 20 21
metanephros
moderate moderate
regionalmoderate expression: see section 08 09 10 11 18 19 20 21 22
lung
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 15 18 19 21 22 weak expression: see section 03 04 16 17 20 23 24
clavicle
strong strong
regionalstrong expression: see section 10 11 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T56005
Entity Detected:Slc38a2, solute carrier family 38, member 2 ( MGI:1915010)
Sequence:sense strand is shown

>T56005
TTGGGTACAACCAATGGTCAGTTCTGTCCCAAGATGAAACCCCTGGGGACTGTGTAGACGCAAAGGCAGT
GAGAAGACACTCCCCCATGCTGCTTACTTGTCATCAAGTCCAGAACCTCACCTGCTCGTCAAAGACTCTG
CACCTCCGCTTGAAGTCCTGGAAAGAGTCCTGGCTACCATATGCCAGGGGATGCTGAGAGGCAGGCATCC
TGAGAGGTGCCATGACAACCAGGATCCTGTTCACAGCCGATCAGTGTGGAGAGACTTCCCAAACCTCCTG
TGAGGGAGCAAGGTTCCAAATGAATTTCAGGCCAAATTCCAAAGCTTGGGGCTGTTTTACCCGCCTTGAG
GTTCCTGAACTGAATTCTCAGCCTTGAGCAGTCTTCAGTATTAAAACCACTGTCCCGTCACCTCAATTCA
CATCGCTGTCTTCCATGGAACGTTTCAATAACTGTACTGTTATTTATAGAGCAGCATTAATCCATTAAAG
CTAACCTATTTTTCAATATTTATGATAAACTGTACATATATTGTCCATATGTATTTGTAAATAGATTGTA
TATACTGTTGGAATTGGGTTCAAGTGTATATATTCCCCTAAGTTTCTTAACTGCATTTTGATGAATTCAC
ATTATATATGTCTCCAAATTACTTGTACAGAGAATTCAATCTTAAGAAATTGACATTGTGTATAATACTA
AAATTGAAAATAACTTGTTTAAATTGTATTTGCAATAAACTGGTTGGACATCAACTTAAGTTCAGATAAT
CTAAAGCTTTAAATGAGGTCTAAATTTTCAAAATGCAGTAGCTACCACATGGCTATCTGGTAACATGTCA
AGTGTGATCTCAATGAAAGAGGACTCCTTGTATGTATATAGTGTAAATGTCCCAGCTGTACTGTTAGAGG
CCAACATGCCTCTGTGGCCTGTTGGCAGAGAGTAAATGCTACACCGGTTTCAATGA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TTGGGTACAACCAATGGTCA; Reverse Primer - name:unspecified, sequence:TCATTGAAACCGGTGTAGCA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:MGI:4828213 same experiment
 EurExpress:euxassay_019685 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS