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EMAGE:995

Pax6 paired box gene 6 ( MGI:97490)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:995
Figure 2L of Bernier et al., 2001 [PMID:11641222] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: ls - lens; rpe - retinal-pigmented epithelium; cm - ciliary margin; nr - neural retina.
Expression Pattern Description
Spatial Annotation:
EMAGE:995EMAGE:995Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
995_voxel_strong_3D_1.wlz
995_voxel_weak_3D_1.wlz
995_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:995_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
pigmented retinal epithelium
detected detected
neural retinal epithelium
detected detected
regionalPredominant expression in the ciliary margin of the neural retina.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2153644
Entity Detected:Pax6, paired box gene 6 ( MGI:97490)
Notes:The Pax6 probe used in this study by Bernier et al., 2001 [PMID:11641222] is described as "800 bp Pax6 cDNA (Walther and Gruss, 1991 [PMID:11641222] )". The exact endpoints of this sequence are unknown.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Bernier et al., 2001 [PMID:11641222] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11641222] Bernier G, Vukovich W, Neidhardt L, Herrmann BG, Gruss P 2001 Isolation and characterization of a downstream target of Pax6 in the mammalian retinal primordium. Development (128):3987-94
 [ PMID:1687460] Walther C, Gruss P 1991 Pax-6, a murine paired box gene, is expressed in the developing CNS. Development (113):1435-49
Links:MGI:2153750 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI