EMAGE mouse gene expression spatial database

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EMAGE:9952

Pus3 pseudouridine synthase 3 ( MGI:1914299)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008161. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008161_01	euxassay_008161_02	euxassay_008161_03	euxassay_008161_04

euxassay_008161_05	euxassay_008161_06	euxassay_008161_07	euxassay_008161_08	euxassay_008161_09

euxassay_008161_10	euxassay_008161_11	euxassay_008161_12	euxassay_008161_13	euxassay_008161_14

euxassay_008161_15	euxassay_008161_16	euxassay_008161_17	euxassay_008161_18	euxassay_008161_19

euxassay_008161_20	euxassay_008161_21	euxassay_008161_22	euxassay_008161_23	euxassay_008161_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9952_wholemount_strong.wlz

9952_wholemount_moderate.wlz

9952_wholemount_weak.wlz

9952_wholemount_possible.wlz

9952_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9952_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

strong

spotted

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 moderate expression: see section 01 02 03 04 05

pituitary gland

strong

spotted

strong expression: see section 11 12 13 14 16 17 18

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 03 weak expression: see section 02

heart ventricle

strong

spotted

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

esophagus

moderate

regional

moderate expression: see section 15 16

tongue muscle

strong

regional

strong expression: see section 11 12

mandible

moderate

regional

moderate expression: see section 02 03 04 05 07 08 19 20 weak expression: see section 06 09 10 17 18 21 22 23 24

maxilla

moderate

regional

moderate expression: see section 02 03 04 05 07 08 20 weak expression: see section 06 10 17 18 21 22 23

urethra of male

strong

regional

strong expression: see section 15 moderate expression: see section 16

lung

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 19 20 22 23 24 moderate expression: see section 15 16 18 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T255
Entity Detected:	Pus3, pseudouridine synthase 3 ( MGI:1914299)
Sequence:	sense strand is shown >T255 TGCCTTTGTAGAAGGAGTGAAAATGCGCACATATAAGCCCCTCATGGATCGTCCCAAGTGCCAAGGACTG GAATCCCGGATCCGGCATTTTGTGAGCAGAGGCAGAATTGAGCACCCACATTTACTCCATAAGGAAGAAA TAAAAGCCAGAAGGGACTGTGCTGACAAAGAAGAAAATACTGTTGTAGAGAATCCAAGTAAGAGGGTTTG TATAATAGATGCAGAAATTAACAGCATTGCATAATCCTAGGAAGCCTAGCATCTCTAACAGGATCCAGGG AGCAATAACCAGTTAAAATTCTAATGATGACAAAAGGGCCTAACAAGTTGTTAAACAGAAAAGGATGTAT TCTCTAATCCCTGTCCAAAAGAGTGATGAAATGAAAGACGCAAAAAGCACT
Notes:	The probe template was PCR amplified from IMAGE:2654123 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2654123 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9951 same embryo
	EMAGE:9950 same embryo
	EurExpress:euxassay_008161 same experiment
	MGI:4827538 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source