EMAGE mouse gene expression spatial database

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EMAGE:9955

Sdr42e1 short chain dehydrogenase/reductase family 42E, member 1 ( MGI:1921282)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000194. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000194_01	euxassay_000194_02	euxassay_000194_03	euxassay_000194_04

euxassay_000194_05	euxassay_000194_06	euxassay_000194_07	euxassay_000194_08	euxassay_000194_09

euxassay_000194_10	euxassay_000194_11	euxassay_000194_12	euxassay_000194_13	euxassay_000194_14

euxassay_000194_15	euxassay_000194_16	euxassay_000194_17	euxassay_000194_18	euxassay_000194_19

euxassay_000194_20	euxassay_000194_21	euxassay_000194_22	euxassay_000194_23	euxassay_000194_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9955_wholemount_strong.wlz

9955_wholemount_moderate.wlz

9955_wholemount_weak.wlz

9955_wholemount_possible.wlz

9955_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9955_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

integumental system muscle

moderate

homogeneous

moderate expression: see section 03 04 06 07 08 09 10 11 12 13 14 15 16 17 20 21 22 23 24

limb

strong

regional

strong expression: see section 01 02 03 18 19 21 22 23 24

axial musculature

moderate

homogeneous

moderate expression: see section 18

axial muscle

moderate

homogeneous

moderate expression: see section 01 02

cranial musculature

moderate

homogeneous

moderate expression: see section 18

central nervous system

strong

regional

strong expression: see section 02 07 08 09 10 11 12 13 14 15 16 17 18 19

skin

strong

homogeneous

strong expression: see section 01 02 04 06 09 10 13 20 21 22 23 24 moderate expression: see section 03 17

epidermis

strong

homogeneous

strong expression: see section 08

vibrissa

strong

homogeneous

strong expression: see section 08

telencephalon

strong

regional

strong expression: see section 01 03 04 06 07 20 21 22 23 24

rest of cerebellum marginal layer

strong

homogeneous

strong expression: see section 03 04 07 08 09 10 11 12 15 16 17 20 21

rest of cerebellum ventricular layer

strong

homogeneous

strong expression: see section 03 04 07 08 09 10 11 12 15 16 17 20 21

metencephalon rest of alar plate marginal layer

strong

regional

strong expression: see section 08 09 10 11 12 13 16 17 20 21

metencephalon rest of alar plate ventricular layer

strong

regional

strong expression: see section 08 09 10 11 12 13 16 17 20 21

liver

strong

spotted

strong expression: see section 04 moderate expression: see section 01 02 03 06 07 09 10 11 12 14 15 16 17 18 20 21 22 23 24

skeleton

strong

regional

strong expression: see section 04 20 21 22 moderate expression: see section 03 07 08 09 10 11 12 13 14 15 16 17 18 23 24

cranium

moderate

regional

moderate expression: see section 01 02

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T210
Entity Detected:	Sdr42e1, short chain dehydrogenase/reductase family 42E, member 1 ( MGI:1921282)
Sequence:	sense strand is shown >T210 CGCAGAGAAGAAGGTCCTGGAAGCCAATGGCTTGGCCTTCAAGCAGGGCGATGGTATACTCCGGACCTGC GCCATCAGGCCAGCCGGCATCTACGGGGCTGGGGAGCAGAGGCATCTTCCCAGGATAGTGAGTTACATTG AGAGGGGCCTGTTCAGATTTGTGTACGGGGATCCCCAGAGCCTGGTTGAGTTTGTCCACGTGGATAACCT GGCGAAGGCTCACATTCTGGCCTCTGAGGCTCTGAAAGCCGATAAAGGCCACGTTGCCTCCGGTCAGCCC TACTTCATCTCAGATGGTAGACCGGTCAATAACTTTGAGTTCTTTAGGCCACTGGTCGAGGGCCTGGGCT ACACATTCCCATCCACCCGCCTGCCCCTAACCCTCATCTACTGCCTTGCTTTCTTGGTAGAGATGACCCA CTTCATTGTGGGCAGGCTCTACAACTTCCAGCCCTTCCTCACCCGCACCGAAGTTTATAAAACTGGCG
Notes:	The probe template was PCR amplified from IMAGE:2648754 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2648754 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9953 same embryo
	EMAGE:9954 same embryo
	EurExpress:euxassay_000194 same experiment
	MGI:4827927 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source