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EMAGE:9962

Wdr16 WD repeat domain 16 ( MGI:1919110)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9962 EMAGE:9962 EMAGE:9962 EMAGE:9962 EMAGE:9962
"Pseudo-wholemount" of euxassay_008152. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008152_01 euxassay_008152_02 euxassay_008152_03 euxassay_008152_04
EMAGE:9962 EMAGE:9962 EMAGE:9962 EMAGE:9962 EMAGE:9962
euxassay_008152_05 euxassay_008152_06 euxassay_008152_07 euxassay_008152_08 euxassay_008152_09
EMAGE:9962 EMAGE:9962 EMAGE:9962 EMAGE:9962 EMAGE:9962
euxassay_008152_10 euxassay_008152_11 euxassay_008152_12 euxassay_008152_13 euxassay_008152_14
EMAGE:9962 EMAGE:9962 EMAGE:9962 EMAGE:9962 EMAGE:9962
euxassay_008152_15 euxassay_008152_16 euxassay_008152_17 euxassay_008152_18 euxassay_008152_19
EMAGE:9962
euxassay_008152_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9962Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9962_wholemount_strong.wlz
9962_wholemount_moderate.wlz
9962_wholemount_weak.wlz
9962_wholemount_possible.wlz
9962_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9962_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
lateral ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
4th ventricle
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T76
Entity Detected:Wdr16, WD repeat domain 16 ( MGI:1919110)
Sequence:sense strand is shown

>T76
GTGTCACTGCCAGCACCGATGGGACATGCATCATTTGGGACCTCGTGCGACTTAGAAGGAATCAGATGAT
CCTGGCCAACACCCTATTCCAGTGTGTTTGTTACCACCCGGAAGAATTCCAGATCATCACCAGTGGCACA
GACAGGAAGATTGCTTACTGGGAAGTATTTGATGGATCAGTAATCAGAGAGTTGGAAGGCTCCTTGTCTG
GGTCCATCAATGGTATGGATATCACTCAGGAAGGAGGGCACTTTGTTACAGGTGGCCATGACCATCTGGT
CAAAGTCTGGGACTACAACGAGGGTGAAGTGACTCACGTCGGGGTGGGGCACAGCGGCAACATCATGGCC
ATGCGGATAAGTCCAGGAAACCAGTATATCGTCAGTGTGAGCGCAGATGGAGCCATCTTGAGGTGGAAAT
ACCCATTTGCTTCGTGATGGGCTCTGGATCTCCGCAGCTTCCACCCTGTGTCGAGCACAGGCCTGTCCAG
GGCCAAGTTTGCATAACTCCAGAACCG
Notes:The probe template was PCR amplified from IMAGE:1135288 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1135288 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9960 same embryo
 EMAGE:9961 same embryo
 EurExpress:euxassay_008152 same experiment
 MGI:4829193 same experiment
 EMAGE:30432 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS