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EMAGE:997

Pax3 paired box gene 3 ( MGI:97487)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:997
Fig 9B(left) of Solloway & Robertson, 1999 [PMID:10079236] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: fb - forelimb bub.
Expression Pattern Description
Spatial Annotation:
EMAGE:997Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
997_wholemount_strong_3D_1.wlz
997_wholemount_moderate_3D_1.wlz
997_wholemount_weak_3D_1.wlz
997_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:997_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalExpression is restricted to the dermomyotome.
neural tube
detected detected
regionalPax3 transcripts are also localised along the length of the dorsal neural tube.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1101193
Entity Detected:Pax3, paired box gene 3 ( MGI:97487)
Sequence:sense strand is shown

>MGI:1101193
AAGCTTACCGAGGCCCGAGTGCAGGTCTGGTTTAGCAACCGCCGTGCAAGATGGAGGAAACAAGCTGGAG
CCAATCAACTGATGGCTTTCAACCATCTCATTCCGGGGGGATTCCCTCCCACCGCCATGCCGACCCTGCC
AACATACCAGCTGTCGGAGACCTCTTACCAGCCCACGTCTATTCCACAAGCCGTGTCAGATCCCAGTAGC
ACCGTCCACAGACCTCAGCCGCTTCCTCCGAGCACTGTACACCAAAGCACTATTCCTTCGAACGCAGACA
GCAGCTCTGCCTACTGCCTCCCCAGCACCAGGCATGGATTTTCAAGCTATACAGACAGCTTTGTGCCTCC
ATCGGGGCCCTCCAACCCCATGAACCCCACCATCGGCAATGGCCTTTCACCTCAGGTAATGGGACTTCTG
ACCAACCACGGTGGGGTACCGCACCAGCCTCAGACCGACTATGCTCTCTCCCCTCTCACTGGGGGCCTGG
AACCCACGACCACGGTGTCAGCCAGCTGCA
nt 1157 - nt 1676 of NM_008781.2
Notes:The Pax3 probe used in this study by Solloway & Robertson, 1999 [PMID:10079236] was previously described by Goulding et al., 1991 [PMID:2022185] as "a 516 bp Hind-PstI fragment from the 3' end of the Pax-3 gene." Restriction analysis of GenBank seq NM_008781.2 with HindIII and PstI reveals a fragment of suitable size (ie. nt 1157-1676 as noted above).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Solloway & Robertson, 1999 [PMID:10079236] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10079236] Solloway MJ, Robertson EJ 1999 Early embryonic lethality in Bmp5;Bmp7 double mutant mice suggests functional redundancy within the 60A subgroup. Development (126):1753-68
 [ PMID:2022185] Goulding MD, Chalepakis G, Deutsch U, Erselius JR, Gruss P 1991 Pax-3, a novel murine DNA binding protein expressed during early neurogenesis. EMBO J (10):1135-47
Links:MGI:1333145 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI