EMAGE mouse gene expression spatial database

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EMAGE:9991

Slc7a5 solute carrier family 7 (cationic amino acid transporter, y+ system), member 5 ( MGI:1298205)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019697. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019697_01	euxassay_019697_02	euxassay_019697_03	euxassay_019697_04

euxassay_019697_05	euxassay_019697_06	euxassay_019697_07	euxassay_019697_08	euxassay_019697_09

euxassay_019697_10	euxassay_019697_11	euxassay_019697_12	euxassay_019697_13	euxassay_019697_14

euxassay_019697_15	euxassay_019697_16	euxassay_019697_17	euxassay_019697_18	euxassay_019697_19

euxassay_019697_20	euxassay_019697_21	euxassay_019697_22	euxassay_019697_23	euxassay_019697_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9991_wholemount_strong.wlz

9991_wholemount_moderate.wlz

9991_wholemount_weak.wlz

9991_wholemount_possible.wlz

9991_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9991_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand mesenchyme

weak

regional

weak expression: see section 22 23 24

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 17 18 19 20 21 22 23 24

vibrissa

weak

regional

weak expression: see section 04 05 06 07 20 21 22

lower jaw incisor

moderate

regional

moderate expression: see section 10 weak expression: see section 11 12 15 16 17

upper jaw incisor

moderate

regional

moderate expression: see section 10 weak expression: see section 11 12 15 16 17

testis

strong

regional

strong expression: see section 06 07 08 21 22 23 moderate expression: see section 09

clavicle

moderate

regional

moderate expression: see section 10 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T56018
Entity Detected:	Slc7a5, solute carrier family 7 (cationic amino acid transporter, y+ system), member 5 ( MGI:1298205)
Sequence:	sense strand is shown >T56018 AGCCATGACCCTAACAGCACCTAGCTGTCCGCTGCAGGGCCTTGTGCTCTGTGCACACAATGCAGCAGTG CCCCGGCTCTCACACGCACAGCACTCCCCGCCCTGCCCCACCTCTCTTAGAACCAGACCAGCACCATGGC TCTCCCACCCCCACCCCCACCCCCAAGCACCTGGTCTGTCCCCATTGTTCTTGAGACTGCTAGCAGCATT CTGTCAAAGTCACTACTGCTCCCTCCAGCCCCACCCTTAGCCATTGTTCAGAAAAATAGGCACCAAGCAC CCCTCTGCTCTCTGCCACCAGTGTGGTGTGACCACTCCAGCTCCCTGAGTATGAAAGCCACTGGCTACAT GTGTGGGACCATCCTGCAGCCTTCCCGTTGTGCTCTGGGTGAGACAGTTGGGTCCAGCCAGCACATCACT GAAGGAGCTGATCTGCTGGAGGGACTTCAGGATGTGCGTCCCCTGCTGGCCTCTGCCATTGCCTCAGACC ATGGCCTCCCTGAAGGGGTGTCTACTGGAATGGCTACTATGCAGTTCTTACATAGTGGTGACTTACCACT GGCCACCCTGACCTCTCCTCTGTGCTTCTGGGGTTCCCTTTCATTGGGGTGAGGAAGCAAGGTCAAGGTC AGAGTGCCTCTTGAAGGTTCTCCCAGATATGGGAACTGGGGCCTCTTCCCTGGAAATGAGGGCAGGGACC CAGTTCCATCCTGGGGTGTGTCCCCAGATACAGCCTGGGTTAAAGGGCAGGGATTCATGGTGCTGACCCA GGCTCTCTGAGAAGGCCCTGTCATTCCTGTTTGTGATGTTCTGAGGATGCCCATCTGTAGGTTTTTATTT TCAAATCAAGCTCCGACCACCGCCATTTTGCCCAGTGCTTTCTATTTTGGTTTTATCCTAACATGTCACT GCCCTGAAAGACACCCCTACCTGGCCCCTTTCAATGGTCAGTGTCTGACCCACCACACTGGGTCCATCTG CTGTGCCACCTGAGATA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AGCCATGACCCTAACAGCAC; Reverse Primer - name:unspecified, sequence:TATCTCAGGTGGCACAGCAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4828280 same experiment
	EurExpress:euxassay_019697 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source